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Validation Data Gallery

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  • Western Blot (WB) analysis of MCF-7 cells using GATA3 Polyclonal antibody (22343-1-AP)

    WB analysis of MCF-7 using 22343-1-AP (same clone as 22343-1-PBS)

    WB result of GATA3 antibody (22343-1-AP; 1:3000; incubated at room temperature for 1.5 hours) with sh-Control and sh-GATA3 transfected MCF-7 cells. This data was developed using the same antibody clone with 22343-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of mouse thymus tissue using GATA3 Polyclonal antibody (22343-1-AP)

    WB analysis of mouse thymus using 22343-1-AP (same clone as 22343-1-PBS)

    mouse thymus tissue were subjected to SDS PAGE followed by western blot with 22343-1-AP (GATA3 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 22343-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using GATA3 Polyclonal antibody (22343-1-AP)

    WB analysis using 22343-1-AP (same clone as 22343-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 22343-1-AP (GATA3 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 22343-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of MCF-7 cells using GATA3 Polyclonal antibody (22343-1-AP)

    IF Staining of MCF-7 using 22343-1-AP (same clone as 22343-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using GATA3 antibody (22343-1-AP) at dilution of 1:600 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), Alpha Tubulin antibody (66031-1-Ig, Clone: 1E4C11, red). This data was developed using the same antibody clone with 22343-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO Validated

GATA3 Polyclonal antibody, PBS Only

GATA3 Polyclonal Antibody for WB, IF/ICC, Indirect ELISA
Cat No. 22343-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IF/ICC, Indirect ELISA

Synonyms

GATA binding factor 3, GATA-binding factor 3, Trans-acting T-cell-specific transcription factor GATA-3

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

22343-1-PBS targets GATA3 in WB, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag17833

Product name: Recombinant human GATA3 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-262 aa of BC006793

Sequence: MEVTADQPRWVSHHHPAVLNGQHPDTHHPGLSHSYMDAAQYPLPEEVDVLFNIDGQGNHVPPYYGNSVRATVQRYPPTHHGSQVCRPPLLHGSLPWLDGGKALGSHHTASPWNLSPFSKTSIHHGSPGPLSVYPPASSSSLSGGHASPHLFTFPPTPPKDVSPDPSLSTPGSAGSARQDEKECLKYQVPLPDSMKLESSHSRGSMTALGGASSSTHHPITTYPPYVPEYSSGLFPPSSLLGGSPTGFGCKSRPKARSSTEGR

相同性解析による交差性が予測される生物種
Full Name GATA binding protein 3
Calculated molecular weight 443 aa, 48 kDa
Observed molecular weight 37 kDa and 48 kDa
GenBank accession numberBC006793
Gene Symbol GATA3
Gene ID (NCBI) 2625
RRIDAB_2879082
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDP23771
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

Trans-acting T-cell-specific transcription factor, also known as GATA-3, belongs to the zinc-finger transcription factor GATA family which drives the differentiation of T helper (Th) 2 cells. GATA factors are expressed in a tissue-specific manner and GATA3 is present in both hematopoietic (e.g., T cells) and non-hematopoietic tissues, including the kidney, central nervous system, skin and mammary gland (PMID: 25148023). GATA-3 plays a central role in Th2 responses both in vitro and in vivo (PMID: 16467870). The full length GATA3 protein contains 444 aa corresponding to the molecular weights of 48,0 kDa, and the truncated protein was deteced at approximately 37 kDa (PMID: 24758297).