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- KD/KO Validated
GATA3 Polyclonal antibody
GATA3 Polyclonal Antibody for WB, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human
Applications
WB, ChIP, ELISA
Conjugate
Unconjugated
Cat no : 10417-1-AP
Synonyms
Validation Data Gallery
Tested Applications
| Positive WB detected in | MCF-7 cells |
| Positive IHC detected in | human breast cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Sample-dependent, check data in validation data gallery | |
Published Applications
| KD/KO | See 2 publications below |
| WB | See 19 publications below |
| ChIP | See 1 publications below |
Product Information
10417-1-AP targets GATA3 in WB, ChIP, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Cited Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | GATA3 fusion protein Ag0174 相同性解析による交差性が予測される生物種 |
| Full Name | GATA binding protein 3 |
| Calculated molecular weight | 443 aa, 48 kDa |
| Observed molecular weight | 45-50 kDa |
| GenBank accession number | BC003070 |
| Gene symbol | GATA3 |
| Gene ID (NCBI) | 2625 |
| RRID | AB_2263174 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
GATA3 is a transcriptional factor that binds to the enhancer elements of all 4 T-cell antigen receptor genes. GATA3 is highly expressed in naive, freshly activated cells and Th2 lineage cells and thought to be necessary and sufficient for Th2 cytokine gene expression. GATA3 and TBET interfer with each other to regulate gene expression. This is a rabbit polyclonal antibody raised against part of C-terminus of human GATA3.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for GATA3 antibody 10417-1-AP | Download protocol |
| IHC protocol for GATA3 antibody 10417-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Cancer Cell Lunatic fringe deficiency cooperates with the Met/Caveolin gene amplicon to induce basal-like breast cancer. | ||
Cancer Res Cooperation between Pik3ca and p53 mutations in mouse mammary tumor formation. | ||
Oxid Med Cell Longev Androgen Plays a Potential Novel Hormonal Therapeutic Role in Th17 Cells Predominant Neutrophilic Severe Asthma by Attenuating BECs Regulated Th17 Cells Differentiation via MBD2 Expression | ||
Cell Death Dis Calreticulin promotes EGF-induced EMT in pancreatic cancer cells via Integrin/EGFR-ERK/MAPK signaling pathway. | ||
Development The inhibitor of histone acetyltransferases Nir regulates epidermis development. | ||
Int J Cardiol Endothelial microparticles exert differential effects on functions of Th1 in patients with acute coronary syndrome. |
