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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using GAT1 Recombinant antibody (85060-2-RR)

    WB analysis using 85060-2-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 85060-2-RR (SLC6A1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of mouse cerebellum tissue using GAT1 Recombinant antibody (85060-2-RR)

    IHC staining of mouse cerebellum using 85060-2-RR

    Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 85060-2-RR (GAT1 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse cerebellum tissue using GAT1 Recombinant antibody (85060-2-RR)

    IHC staining of mouse cerebellum using 85060-2-RR

    Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 85060-2-RR (GAT1 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of rat brain tissue using GAT1 Recombinant antibody (85060-2-RR)

    IHC staining of rat brain using 85060-2-RR

    Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 85060-2-RR (GAT1 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of rat brain tissue using GAT1 Recombinant antibody (85060-2-RR)

    IF Staining of rat brain using 85060-2-RR

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded rat brain tissue using GAT1 antibody (85060-2-RR, Clone: 242637D11 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Affinity and Kinetic Characterization of 85060-2-RR

    Affinity and Kinetic Characterization of 85060-2-RR

    Biolayer interferometry (BLl) kinetic assays of 85060-2-RR against Human GAT1 were performed. The affinity constant is below 1 pM.

GAT1 Recombinant monoclonal antibody

GAT1 Uni-rAb® Recombinant Antibody for WB, IHC, IF-P, ELISA
Cat No. 85060-2-RR
Clone No.242637D11

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF-P, ELISA

Synonyms

SLC6A1, 242637D11, GABA Transporter 1, GABATHG, GABATR

Formulation:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

ご購入について

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国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター（国内）に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。

保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inunboiled rat brain tissue
Positive IHC detected inmouse cerebellum tissue, rat brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected inrat brain tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:200-1:800
Immunofluorescence (IF)-PIF-P : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

85060-2-RR targets GAT1 in WB, IHC, IF-P, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen

CatNo: Ag29712

Product name: Recombinant human SLC6A1 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-52 aa of BC033904

Sequence: MATNGSKVADGQISTEVSEAPVANDKPKTLVVKVQKKAADLPDRDTWKGRFD

相同性解析による交差性が予測される生物種
Full Name solute carrier family 6 (neurotransmitter transporter, GABA), member 1
Calculated molecular weight 67 kDa
Observed molecular weight67 kDa
GenBank accession numberBC033904
Gene Symbol GABA Transporter 1/GAT1
Gene ID (NCBI) 6529
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein A purification
UNIPROT IDP30531
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
IF protocol for GAT1 antibody 85060-2-RRDownload protocol
IHC protocol for GAT1 antibody 85060-2-RRDownload protocol
WB protocol for GAT1 antibody 85060-2-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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