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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using GAT1 Polyclonal antibody (20298-1-AP)

    WB analysis using 20298-1-AP (same clone as 20298-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 20298-1-AP (GAT1 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 20298-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse cerebellum tissue using GAT1 Polyclonal antibody (20298-1-AP)

    IHC staining of mouse cerebellum using 20298-1-AP (same clone as 20298-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 20298-1-AP (GAT1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 20298-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse cerebellum tissue using GAT1 Polyclonal antibody (20298-1-AP)

    IHC staining of mouse cerebellum using 20298-1-AP (same clone as 20298-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 20298-1-AP (GAT1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 20298-1-PBS in a different storage buffer formulation.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

20298-1-PBS targets GAT1 in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen GAT1 fusion protein Ag14109 相同性解析による交差性が予測される生物種
Full Name solute carrier family 6 (neurotransmitter transporter, GABA), member 1
Calculated molecular weight 67 kDa
Observed molecular weight 42 kDa, 67 kDa
GenBank accession numberBC033904
Gene Symbol GABA Transporter 1/GAT1
Gene ID (NCBI) 6529
RRIDAB_2878664
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP30531
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.