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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using GALR2 Polyclonal antibody (29909-1-AP)

    WB analysis using 29909-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 29909-1-AP (GALR2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human pancreas cancer tissue using GALR2 Polyclonal antibody (29909-1-AP)

    IHC staining of human pancreas cancer using 29909-1-AP

    Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 29909-1-AP (GALR2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human pancreas cancer tissue using GALR2 Polyclonal antibody (29909-1-AP)

    IHC staining of human pancreas cancer using 29909-1-AP

    Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 29909-1-AP (GALR2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using GALR2 Polyclonal antibody (29909-1-AP)

    IF Staining of HepG2 using 29909-1-AP

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using GALR2 antibody (29909-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

GALR2 Polyclonal antibody

GALR2 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 29909-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, ELISA

Synonyms

GALR 2, GALNR2, Galanin receptor type 2, galanin receptor 2, GAL2-R

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inHepG2 cells, SMMC-7721 cells
Positive IHC detected inhuman pancreas cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

29909-1-AP targets GALR2 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag31762

Product name: Recombinant human GALR2 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 287-387 aa of NM_003857

Sequence: VNPIVYALVSKHFRKGFRTICAGLLGRAPGRASGRVCAAARGTHSGSVLERESSDLLHMSEAAGALRPCPGASQPCILEPCPGPSWQGPKAGDSILTVDVA

相同性解析による交差性が予測される生物種
Full Name galanin receptor 2
Calculated molecular weight42KD
Observed molecular weight 42-50 kDa
GenBank accession numberNM_003857
Gene Symbol GALR2
Gene ID (NCBI) 8811
RRIDAB_2935488
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDO43603
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Galanin receptor type 2 (GALR2) is a receptor for galanin, GALP, and the hormone SPX1 (PMID:24517231). It is an important neuromodulator present in the brain, gastrointestinal system, and hypothalamo-pituitary axis. Activation of the GALR2 may protect the hippocampus from neuronal damage (PMID: 17263796). It is a 30-amino acid non-C-terminally amidated peptide that potently stimulates growth hormone secretion, inhibits cardiac vagal slowing of heart rate, abolishes sinus arrhythmia, and inhibits postprandial gastrointestinal motility. The activity of this receptor is mediated by G proteins that activate the phospholipase C/protein kinase C pathway (via G(q)) and that inhibits adenylyl cyclase. GALR2 has a calculated molecular weight of 42 kDa.

Protocols

Product Specific Protocols
WB protocol for GALR2 antibody 29909-1-APDownload protocol
IHC protocol for GALR2 antibody 29909-1-APDownload protocol
IF protocol for GALR2 antibody 29909-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols