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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

GALR2 Polyclonal antibody

GALR2 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 29909-1-AP

Synonyms

GAL2 R, galanin receptor 2, Galanin receptor type 2, GALNR2, GALR 2, GALR2

Validation Data Gallery

Applications:
  • WB analysis using 29909-1-AP

    WB analysis using 29909-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 29909-1-AP (GALR2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • IHC staining of human pancreas cancer using 29909-1-AP

    IHC staining of human pancreas cancer using 29909-1-AP

    Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 29909-1-AP (GALR2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with GALR2 antibody included
  • IHC staining of human pancreas cancer using 29909-1-AP

    IHC staining of human pancreas cancer using 29909-1-AP

    Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 29909-1-AP (GALR2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with GALR2 antibody included

Tested Applications

Positive WB detected inHepG2 cells, SMMC-7721 cells
Positive IHC detected inhuman pancreas cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IHC experiment? We recommend our IHCeasy GALR2 Ready-To-Use IHC Kit. GALR2 primary antibody included.

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

29909-1-AP targets GALR2 in WB, IHC, ELISA applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen GALR2 fusion protein Ag31762 相同性解析による交差性が予測される生物種
Full Name galanin receptor 2
Calculated molecular weight42KD
Observed molecular weight 42-50 kDa
GenBank accession numberNM_003857
Gene symbol GALR2
Gene ID (NCBI) 8811
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Galanin receptor type 2 (GALR2) is a receptor for galanin, GALP, and the hormone SPX1 (PMID:24517231). It is an important neuromodulator present in the brain, gastrointestinal system, and hypothalamo-pituitary axis. Activation of the GALR2 may protect the hippocampus from neuronal damage (PMID: 17263796). It is a 30-amino acid non-C-terminally amidated peptide that potently stimulates growth hormone secretion, inhibits cardiac vagal slowing of heart rate, abolishes sinus arrhythmia, and inhibits postprandial gastrointestinal motility. The activity of this receptor is mediated by G proteins that activate the phospholipase C/protein kinase C pathway (via G(q)) and that inhibits adenylyl cyclase. GALR2 has a calculated molecular weight of 42 kDa.

Protocols

Product Specific Protocols
WB protocol for GALR2 antibody 29909-1-APDownload protocol
IHC protocol for GALR2 antibody 29909-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols