Validation Data Gallery
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Tested Applications
| Positive WB detected in | HEK-293 cells, human liver tissue |
| Positive IHC detected in | mouse small intestine tissue, human kidney tissue, human testis tissue, human placenta tissue, human brain tissue, human lung tissue, human ovary tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
16900-1-AP targets GALNTL2 in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.
| Tested Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | GALNTL2 fusion protein Ag10434 相同性解析による交差性が予測される生物種 |
| Full Name | UDP-N-acetyl-alpha-D-galactosamine:polypeptide N-acetylgalactosaminyltransferase-like 2 |
| Calculated molecular weight | 639 aa, 73 kDa |
| Observed molecular weight | 65-70 kDa |
| GenBank accession number | BC014789 |
| Gene Symbol | GALNTL2 |
| Gene ID (NCBI) | 117248 |
| RRID | AB_2263208 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q8N3T1 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
GALNTL2, also known as GALNT15, is a member of the polypeptide ppGalNAc-T family. It catalyzes the initial step in O-linked oligosaccharide biosynthesis by transferring an N-acetyl-D-galactosamine residue to serine or threonine residues on protein receptors. Though it shows weaker activity compared to GALNT2, it can transfer up to seven GalNAc residues to the Muc5AC peptide, suggesting cooperative action with other GALNT proteins. GALNTL2 is widely expressed, with high expression in tissues like the small intestine, placenta, spleen, cerebral cortex, and ovary.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for GALNTL2 antibody 16900-1-AP | Download protocol |
| IHC protocol for GALNTL2 antibody 16900-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |