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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using GALNT6 Polyclonal antibody (13671-1-AP)

    WB analysis using 13671-1-AP (same clone as 13671-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 13671-1-AP (GALNT6 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 13671-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human lung squamous cell cancer using GALNT6 Polyclonal antibody (13671-1-AP)

    IHC staining of human lung squamous cell cancer using 13671-1-AP (same clone as 13671-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human lung squamous cell cancer slide using 13671-1-AP (GALNT6 antibody) at dilution of 1:800 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 13671-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human lung squamous cell cancer using GALNT6 Polyclonal antibody (13671-1-AP)

    IHC staining of human lung squamous cell cancer using 13671-1-AP (same clone as 13671-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human lung squamous cell cancer slide using 13671-1-AP (GALNT6 antibody) at dilution of 1:800 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 13671-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human stomach cancer tissue using GALNT6 Polyclonal antibody (13671-1-AP)

    IHC staining of human stomach cancer using 13671-1-AP (same clone as 13671-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 13671-1-AP (GALNT6 antibody) at dilution of 1:800 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 13671-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human stomach cancer tissue using GALNT6 Polyclonal antibody (13671-1-AP)

    IHC staining of human stomach cancer using 13671-1-AP (same clone as 13671-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 13671-1-AP (GALNT6 antibody) at dilution of 1:800 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 13671-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of A431 cells using GALNT6 Polyclonal antibody (13671-1-AP)

    IF Staining of A431 using 13671-1-AP (same clone as 13671-1-PBS)

    Immunofluorescent analysis of (-20°C Methanol) fixed A431 cells using GALNT6 antibody (13671-1-AP) at dilution of 1:400 and CoraLite®594-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-4). This data was developed using the same antibody clone with 13671-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of A431 cells using GALNT6 Polyclonal antibody (13671-1-AP)

    IF Staining of A431 using 13671-1-AP (same clone as 13671-1-PBS)

    Immunofluorescent analysis of (-20°C Methanol) fixed A431 cells using GALNT6 antibody (13671-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), Beta Actin antibody (66009-1-Ig, Clone: 2D4H5, red). This data was developed using the same antibody clone with 13671-1-PBS in a different storage buffer formulation.

GALNT6 Polyclonal antibody, PBS Only

GALNT6 Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 13671-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, Indirect ELISA

Synonyms

EC:2.4.1.41, GALNAC T6, GalNAcT6, GalNAc-T6, Polypeptide N-acetylgalactosaminyltransferase 6

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

13671-1-PBS targets GALNT6 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag4652

Product name: Recombinant human GALNT6 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 323-622 aa of BC035822

Sequence: FDWSLTFGWETLPPHEKQRRKDETYPIKSPTFAGGLFSISKSYFEHIGTYDNQMEIWGGENVEMSFRVWQCGGQLEIIPCSVVGHVFRTKSPHTFPKGTSVIARNQVRLAEVWMDSYKKIFYRRNLQAAKMAQEKSFGDISERLQLREQLHCHNFSWYLHNVYPEMFVPDLTPTFYGAIKNLGTNQCLDVGENNRGGKPLIMYSCHGLGGNQYFEYTTQRDLRHNIAKQLCLHVSKGALGLGSCHFTGKNSQVPKDEEWELAQDQLIRNSGSGTCLTSQDKKPAMAPCNPSDPHQLWLFV

相同性解析による交差性が予測される生物種
Full Name UDP-N-acetyl-alpha-D-galactosamine:polypeptide N-acetylgalactosaminyltransferase 6 (GalNAc-T6)
Calculated molecular weight 622 aa, 71 kDa
Observed molecular weight71 kDa
GenBank accession numberBC035822
Gene Symbol GALNT6
Gene ID (NCBI) 11226
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDQ8NCL4
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

GALNT6 (polypeptide N-acetylgalactosaminyltransferase 6, GalNAc-T6), which is involved in the initiation of O-glycosylation, has been reported to play crucial roles in mammary carcinogenesis through binding to several substrates (PMID: 32393740). GALNT6 is upregulated in breast, ovarian, renal cell, lung and pancreatic cancers (PMID: 20215525). Overexpression of GRP78 could enhance Golgi-to-ER relocation of GALNT6 (PMID: 28110670).

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