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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using GALNT2 Polyclonal antibody (17441-1-AP)

    WB analysis using 17441-1-AP (same clone as 17441-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 17441-1-AP (GALNT2 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 17441-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse pancreas tissue using GALNT2 Polyclonal antibody (17441-1-AP)

    IHC staining of mouse pancreas using 17441-1-AP (same clone as 17441-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue slide using 17441-1-AP (GALNT2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 17441-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human colon tissue using GALNT2 Polyclonal antibody (17441-1-AP)

    IHC staining of human colon using 17441-1-AP (same clone as 17441-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 17441-1-AP (GALNT2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 17441-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using GALNT2 Polyclonal antibody (17441-1-AP)

    IF Staining of HeLa using 17441-1-AP (same clone as 17441-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using GALNT2 antibody (17441-1-AP) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002), CL594-Phalloidin (red). This data was developed using the same antibody clone with 17441-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using GALNT2 Polyclonal antibody (17441-1-AP)

    IF Staining of HeLa using 17441-1-AP (same clone as 17441-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using GALNT2 antibody (17441-1-AP) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002), CL594-Phalloidin (red). This data was developed using the same antibody clone with 17441-1-PBS in a different storage buffer formulation.

GALNT2 Polyclonal antibody, PBS Only

GALNT2 Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 17441-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, Indirect ELISA

Synonyms

pp GaNTase 2, Polypeptide N-acetylgalactosaminyltransferase 2 soluble form, Polypeptide N-acetylgalactosaminyltransferase 2, GalNAc-T2, GalNAc T2

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

17441-1-PBS targets GALNT2 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag11523

Product name: Recombinant human GALNT2 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 222-571 aa of BC041120

Sequence: FLDSHCECNEHWLEPLLERVAEDRTRVVSPIIDVINMDNFQYVGASADLKGGFDWNLVFKWDYMTPEQRRSRQGNPVAPIKTPMIAGGLFVMDKFYFEELGKYDMMMDVWGGENLEISFRVWQCGGSLEIIPCSRVGHVFRKQHPYTFPGGSGTVFARNTRRAAEVWMDEYKNFYYAAVPSARNVPYGNIQSRLELRKKLSCKPFKWYLENVYPELRVPDHQDIAFGALQQGTNCLDTLGHFADGVVGVYECHNAGGNQEWALTKEKSVKHMDLCLTVVDRAPGSLIKLQGCRENDSRQKWEQIEGNSKLRHVGSNLCLDSRTAKSGGLSVEVCGPALSQQWKFTLNLQQ

相同性解析による交差性が予測される生物種
Full Name UDP-N-acetyl-alpha-D-galactosamine:polypeptide N-acetylgalactosaminyltransferase 2 (GalNAc-T2)
Calculated molecular weight 571 aa, 65 kDa
Observed molecular weight64 kDa
GenBank accession numberBC041120
Gene Symbol GALNT2
Gene ID (NCBI) 2590
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDQ10471
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

GALNT2, a GalNAc-transferase involved in O-linked glycosylation, modulates the risk of atherogenic dyslipidemia, a specific hallmark of insulin resistance and acts as a positive modulator of insulin signaling in human liver cells by down-regulating ENPP1. GALNT2 gene encodes GalNAc-T2 as a regulator of high-density lipoprotein cholesterol (HDL-C) metabolism (PMID: 31040393, 27508872). Western blot analysis detected GALNT2 at an apparent molecular mass of 64 kDa.

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