Validation Data Gallery
Tested Applications
| Positive WB detected in | A549 cells |
| Positive IHC detected in | mouse testis tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
24604-1-AP targets GALNT13 in WB, IHC, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag16526 Product name: Recombinant human GALNT13 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 499-561 aa of BC101032 Sequence: MRGNQLWEYDAESCLSVNKVADGSQHPTVETCNDSTLQKWLLRNYTRMEIFRNIFGNSTDYIL 相同性解析による交差性が予測される生物種 |
| Full Name | UDP-N-acetyl-alpha-D-galactosamine:polypeptide N-acetylgalactosaminyltransferase 13 (GalNAc-T13) |
| Calculated molecular weight | 561 aa, 65 kDa |
| Observed molecular weight | 64 kDa |
| GenBank accession number | BC101032 |
| Gene Symbol | GALNT13 |
| Gene ID (NCBI) | 114805 |
| RRID | AB_3742118 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q8IUC8 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
Polypeptide N-acetylgalactosaminyltransferase 13 (GALNT13) is a recently identified member of the UDP-N-acetylgalactosamine: polypeptide N-acetyl galactosaminy transferases family, otherwise known as ppGalNAc-Tases, which control the initiation of mucin-type O-glycosylation. GALNT13 and the trimeric Tn antigen have been considered as tumor-associated antigens, and might be useful prognostic factors in many types of human cancers (PMID: 34830771, 27499036, 36575396)
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for GALNT13 antibody 24604-1-AP | Download protocol |
| WB protocol for GALNT13 antibody 24604-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |