Validation Data Gallery
Tested Applications
| Positive WB detected in | HaCaT cells, U-87 MG cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
16415-1-AP targets GALNT12 in WB, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag9571 Product name: Recombinant human GALNT12 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-272 aa of BC013945 Sequence: MAGGLFAVSKKYFEYLGSYDTGMEVWGGENLEFSFRIWQCGGVLETHPCSHVGHVFPKQAPYSRNKALANSVRAAEVWMDEFKELYYHRNPRARLEPFGDVTERKQLRDKLQCKDFKWFLETVYPELHVPEDRPGFFGMLQNKGLTDYCFDYNPPDENQIVGHQVILYLCHGMGQNQFFEYTSQKEIRYNTHQPEGCIAVEAGMDTLIMHLCEETAPENQKFILQEDGSLFHEQSKKCVQAARKESSDSFVPLLRDCTNSDHQKWFFKERML 相同性解析による交差性が予測される生物種 |
| Full Name | UDP-N-acetyl-alpha-D-galactosamine:polypeptide N-acetylgalactosaminyltransferase 12 (GalNAc-T12) |
| Calculated molecular weight | 581 aa, 67 kDa |
| Observed molecular weight | 67 kDa |
| GenBank accession number | BC013945 |
| Gene Symbol | GALNT12 |
| Gene ID (NCBI) | 79695 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q8IXK2 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
Polypeptide N‑acetylgalactosaminyl transferase 12 (GALNT12) belongs to the uridine diphosphate N‑acetylgalactosamine gene family, which is involved in numerous biological processes of diseases, such as tumor progression. GALNT12 is highly expressed in the digestive tract and it is frequently downregulated in colorectal cancer (CRC) (PMID: 29095867). Genetic knockdown (KD) and knockout (KO) of GALNT12 in U87 MG, a representative GBM cell line with high GALNT12 expression, confirmed that GALNT12 deficiency leads to decreased cell proliferation, migration and invasion (PMID: 35461073).
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for GALNT12 antibody 16415-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |