Sandwich ELISA standard curve of MP00921-2, Human IL-5 Recombinant Matched Antibody Pair - PBS only. 83962-2-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Eg0380. 83962-1-PBS was biotinylated conjugated as the detection antibody. Range: 7.81-500 pg/mL.

Serum of sixteen individual healthy human donors was measured. All samples were less than the lowest human IL-5 standard, 7.81 pg/mL.

Urine of sixteen individual healthy human donors was measured. All samples were less than the lowest human IL-5 standard, 7.81 pg/mL.

Human peripheral blood mononuclear cells (PBMC) were cultured unstimulated or stimulated with 10 μg/mL PHA for 3 days or 5 days. For the PBMC supernatant cultured for 3 days, the mean IL-5 concentration was determined to be 3.6 pg/mL in unstimulated PBMC supernatant, 377.4 pg/mL in PHA stimulated PBMC supernatant. For the PBMC supernatant cultured for 5 days, the mean IL-5 concentration was determined to be 15.6 pg/mL in unstimulated PBMC supernatant, 332.4 pg/mL in PHA stimulated PBMC supernatant.

Cytometric bead array standard curve of MP00920-1, GALNT10 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 83962-1-PBS. Detection antibody: 83962-2-PBS. Standard: Ag14751. Range: 0.625-80 ng/mL.