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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using GALNT1 Polyclonal antibody (21139-1-AP)

    WB analysis using 21139-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 21139-1-AP (GALNT1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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GALNT1 Polyclonal antibody

GALNT1 Polyclonal Antibody for WB, ELISA
Cat No. 21139-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, ELISA

Synonyms

GALNAC T1, GALNT1, pp GaNTase 1

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inBGC-823 cells, HepG2 cells, HuH-7 cells, MKN-45 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

21139-1-AP targets GALNT1 in WB, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen GALNT1 fusion protein Ag15591 相同性解析による交差性が予測される生物種
Full Name UDP-N-acetyl-alpha-D-galactosamine:polypeptide N-acetylgalactosaminyltransferase 1 (GalNAc-T1)
Calculated molecular weight 559 aa, 64 kDa
Observed molecular weight64 kDa
GenBank accession numberBC038440
Gene Symbol GALNT1
Gene ID (NCBI) 2589
RRIDAB_3085639
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ10472
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

N-acetylgalactosaminyltransferase 1 (GALNT1) is a member of a large family of Golgi resident polypeptide GALNT that initiate mucin-type O-glycosylation by transfer of α-GalNAc from UDP-GalNAc to serine or threonine residues of proteins. GALNT1 is frequently up-regulated in HCC and gastric cancer, and higher GALNT1 expression levels are associated with poorer overall survival (PMID: 25730904, 36439876). The antibody against GALNT1 recognized a specific band at 64 kDa.

Protocols

Product Specific Protocols
WB protocol for GALNT1 antibody 21139-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols