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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

GALNS Polyclonal antibody

GALNS Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IF, IHC, ELISA

Conjugate

Unconjugated

Cat no : 14933-1-AP

Synonyms

Chondroitinase, Chondroitinsulfatase, Galactose 6 sulfate sulfatase, GALNAC6S, GalNAc6S sulfatase, GALNS, GAS, MPS4A

Validation Data Gallery

Applications:
  • WB analysis using 14933-1-AP

    WB analysis using 14933-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 14933-1-AP (GALNS antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • WB analysis of human liver using 14933-1-AP

    WB analysis of human liver using 14933-1-AP

    human liver tissue were subjected to SDS PAGE followed by western blot with 14933-1-AP (GALNS antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

  • IHC staining of human liver cancer using 14933-1-AP

    IHC staining of human liver cancer using 14933-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 14933-1-AP (GALNS antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human liver cancer using 14933-1-AP

    IHC staining of human liver cancer using 14933-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 14933-1-AP (GALNS antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Tested Applications

Positive WB detected inMCF-7 cells, human liver tissue, HepG2 cells, HeLa cells
Positive IHC detected inhuman liver cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

14933-1-AP targets GALNS in WB, IF, IHC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen GALNS fusion protein Ag6746 相同性解析による交差性が予測される生物種
Full Name galactosamine (N-acetyl)-6-sulfate sulfatase
Calculated molecular weight 58 kDa
Observed molecular weight 50-55 kDa
GenBank accession numberBC050684
Gene symbol GALNS
Gene ID (NCBI) 2588
RRIDAB_2108720
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

GALNS(N-acetylgalactosamine-6-sulfatase) is also named as chondroitinase and belongs to the sulfatase family. It is one of sulfatases required to degrade glycosaminoglycans (GAGs), keratan sulfate (KS) and chondroitin-6-sulfate (C6S) and the enzyme is a dimer derived from two 60 kDa polypeptides, each of which is processed to 40 kDa and 15 kDa polypeptide subunits linked by disulfide bonds(PMID:15241807). The deduced 522-residue protein is composed of a 26-amino acid N-terminal signal peptide and a mature polypeptide of 496 amino acid residues, including 2 potential asparagine-linked glycosylation sites(PMID:1755850). Defects in GALNS are the cause of mucopolysaccharidosis type 4A (MPS4A), also known as Morquio A syndrome.

Protocols

Product Specific Protocols
WB protocol for GALNS antibody 14933-1-APDownload protocol
IHC protocol for GALNS antibody 14933-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanIHC,IF

Discov Oncol

Identification of galactosamine-(N-acetyl)-6-sulfatase (GALNS) as a novel therapeutic target in progression of nasopharyngeal carcinoma

Authors - Jin Zhang
View Article