Validation Data Gallery

Filter: AllWBIHCIF/ICC

WB analysis of HepG2 using 11858-1-AP
WB result of Galectin-1 antibody (11858-1-AP; 1:20000; incubated at room temperature for 1.5 hours) with sh-Control and sh-Galectin-1 transfected HepG2 cells.
WB analysis using 11858-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 11858-1-AP (Galectin-1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.
WB analysis using 11858-1-AP
A549 cells lysate was subjected to SDS PAGE followed by western blot with 11858-1-AP (Galectin-1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.
WB analysis using 11858-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 11858-1-AP (Galectin-1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.
IHC staining of human colon cancer using 11858-1-AP
Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 11858-1-AP (Galectin-1 antibody at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human colon using 11858-1-AP
Immunohistochemical analysis of paraffin-embedded human normal colon slide using 11858-1-AP (Galectin-1 antibody) at dilution of 1:1200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human colon cancer using 11858-1-AP
Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 11858-1-AP (Galectin-1 antibody) at dilution of 1:2000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of mouse colon using 11858-1-AP
Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 11858-1-AP (Galectin-1 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of mouse colon using 11858-1-AP
Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 11858-1-AP (Galectin-1 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human lung cancer using 11858-1-AP
Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 11858-1-AP (Galectin-1 antibody at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human lung cancer using 11858-1-AP
Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 11858-1-AP (Galectin-1 antibody at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IF Staining of A549 using 11858-1-AP
Immunofluorescent analysis of (-20°C Ethanol) fixed A549 cells using Galectin-1 antibody (11858-1-AP) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002).

Featured Product
KD/KO Validated

Galectin-1 Polyclonal antibody

Galectin-1 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Cat No. 11858-1-AP

Publications(33)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, ELISA

Synonyms

Beta-galactoside-binding lectin L-14-I, Gal 1, GAL1, Gal-1, Galaptin

Formulation: PBS, Azide, Glycerol

PBS, Azide, Glycerol

Conjugate: Unconjugated

Unconjugated

CoraLite®594

Size/Concentration:

-/ -

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Overview

Tested Applications
Product Information
Publications (33)
Protocols

データシート［PDF］を印刷

MSDS

Tested Applications

Positive WB detected in	A549 cells, HepG2 cells, HCT 116 cells, MCF-7 cells, U2OS cells, NIH/3T3 cells, PC-12 cells
Positive IHC detected in	human colon cancer tissue, human lung cancer tissue, mouse colon tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF/ICC detected in	A549 cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:5000-1:50000
Immunohistochemistry (IHC)	IHC : 1:1000-1:4000
Immunofluorescence (IF)/ICC	IF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

KD/KO	See 1 publications below
WB	See 19 publications below
IHC	See 14 publications below
IF	See 5 publications below
IP	See 2 publications below

Product Information

11858-1-AP targets Galectin-1 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human, mouse, rat
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag2432 Product name: Recombinant human Galectin 1 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-135 aa of BC020675 Sequence: MACGLVASNLNLKPGECLRVRGEVAPDAKSFVLNLGKDSNNLCLHFNPRFNAHGDANTIVCNSKDGGAWGTEQREAVFPFQPGSVAEVCITFDQANLTVKLPDGYEFKFPNRLNLEAINYMAADGDFKIKCVAFD 相同性解析による交差性が予測される生物種
Full Name	lectin, galactoside-binding, soluble, 1
Calculated molecular weight	15 kDa
Observed molecular weight	10-15 kDa
GenBank accession number	BC020675
Gene Symbol	Galectin-1
Gene ID (NCBI)	3956
RRID	AB_2234673
Conjugate	Unconjugated
Form
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	P09382
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

Galectins are a family of animal lectins defined by shared characteristic amino-acid sequences and affinity for β-galactose-containing oligosac-charides (PMID: 8063692). Galectin-1 contains one carbohydrate recognition domain (CRD) and occurs as a monomer as well as a non-covalent homodimer (PMID: 16840800). It is differentially expressed by various normal and pathological tissues. Galectin-1 is a multifunctional protein that is involved in cell adhesion, migration, proliferation, apoptosis, inflammation, tumour transformation and growth (PMID:15785741).

Protocols

Product Specific Protocols
IF protocol for Galectin-1 antibody 11858-1-AP	Download protocol
IHC protocol for Galectin-1 antibody 11858-1-AP	Download protocol
WB protocol for Galectin-1 antibody 11858-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
human	IF	Adv Mater A Hierarchical Plug-and-Supply Flap (PASF) for Instant Perfusion and Oxygen Delivery in Large Soft Tissue Repair. Authors - Zhencheng Liao View Article
human	WB,IP	Cell Mol Immunol Oxidized galectin-1 in SLE fails to bind the inhibitory receptor VSTM1 and increases reactive oxygen species levels in neutrophils Authors - Xu Jiang View Article
human	WB	Bioact Mater Biomimetic nanoparticles drive the mechanism understanding of shear-wave elasticity stiffness in triple negative breast cancers to predict clinical treatment Authors - Dongdong Zheng View Article
rat,human	IHC	Nat Commun Galectin-3-integrin α5β1 phase separation disrupted by advanced glycation end-products impairs diabetic wound healing in rodents. Authors - Zhongyu Zhang View Article
human	IHC,IF	J Exp Clin Cancer Res Nuclear FAM289-Galectin-1 interaction controls FAM289-mediated tumor promotion in malignant glioma. Authors - Xing Rong Guo View Article
mouse,human	WB,IP,IHC	Cancer Lett Cancer-associated fibroblasts promote immune evasion in pancreatic cancer via miR-181b-5p/STING/LGALS1 pathway. Authors - Shaobo Zhang View Article

Validation Data Gallery

WB analysis of HepG2 using 11858-1-AP

WB analysis using 11858-1-AP

WB analysis using 11858-1-AP

WB analysis using 11858-1-AP

IHC staining of human colon cancer using 11858-1-AP

IHC staining of human colon using 11858-1-AP

IHC staining of human colon cancer using 11858-1-AP

IHC staining of mouse colon using 11858-1-AP

IHC staining of mouse colon using 11858-1-AP

IHC staining of human lung cancer using 11858-1-AP

IHC staining of human lung cancer using 11858-1-AP

IF Staining of A549 using 11858-1-AP

Galectin-1 Polyclonal antibody

Galectin-1 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

-/ -

Tested Applications

Recommended dilution

Published Applications

Product Information

Background Information

Protocols

Publications

A Hierarchical Plug-and-Supply Flap (PASF) for Instant Perfusion and Oxygen Delivery in Large Soft Tissue Repair.

Oxidized galectin-1 in SLE fails to bind the inhibitory receptor VSTM1 and increases reactive oxygen species levels in neutrophils

Biomimetic nanoparticles drive the mechanism understanding of shear-wave elasticity stiffness in triple negative breast cancers to predict clinical treatment

Galectin-3-integrin α5β1 phase separation disrupted by advanced glycation end-products impairs diabetic wound healing in rodents.

Nuclear FAM289-Galectin-1 interaction controls FAM289-mediated tumor promotion in malignant glioma.

Cancer-associated fibroblasts promote immune evasion in pancreatic cancer via miR-181b-5p/STING/LGALS1 pathway.

Able™

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