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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

GAK Polyclonal antibody

GAK Polyclonal Antibody for WB, IF, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IF, IHC, ELISA

Conjugate

Unconjugated

Cat no : 12147-1-AP

Synonyms

cyclin G associated kinase, GAK

Validation Data Gallery

Applications:
  • WB analysis of human brain using 12147-1-AP

    WB analysis of human brain using 12147-1-AP

    human brain tissue were subjected to SDS PAGE followed by western blot with 12147-1-AP (GAK antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse brain using 12147-1-AP

    IHC staining of mouse brain using 12147-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 12147-1-AP (GAK antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with GAK antibody included
  • IHC staining of mouse brain using 12147-1-AP

    IHC staining of mouse brain using 12147-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 12147-1-AP (GAK antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with GAK antibody included
  • IF Staining of HepG2 using 12147-1-AP

    IF Staining of HepG2 using 12147-1-AP

    Immunofluorescent analysis of (-20℃ Ethanol) fixed HepG2 cells using 12147-1-AP (GAK antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Tested Applications

Positive WB detected inhuman brain tissue
Positive IHC detected inmouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells
Planning an IHC experiment? We recommend our IHCeasy GAK Ready-To-Use IHC Kit. GAK primary antibody included.

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:250-1:1000
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Published Applications

WBSee 1 publications below

Product Information

12147-1-AP targets GAK in WB, IF, IHC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen GAK fusion protein Ag2795 相同性解析による交差性が予測される生物種
Full Name cyclin G associated kinase
Calculated molecular weight 134 kDa, 143 kDa
Observed molecular weight 150 kDa
GenBank accession numberBC008668
Gene symbol GAK
Gene ID (NCBI) 2580
RRIDAB_2108368
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

GAK(cyclin-G-associated kinase) belongs to the protein kinase superfamily. GAK plays a more positive role as a modulator of signal transduction, by relaying the phosphorylation signal from an unidentified tyrosine kinase to unidentified GAK phosphorylation targets(PMID:9013862). It may be acting as an auxilin homolog that is involved in the uncoating of clathrin- coated vesicles by hsc70 in non-neuronal cells.

Protocols

Product Specific Protocols
WB protocol for GAK antibody 12147-1-APDownload protocol
IHC protocol for GAK antibody 12147-1-APDownload protocol
IF protocol for GAK antibody 12147-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

J Biol Chem

Development of the non-receptor tyrosine kinase FER-targeting PROTACs as a potential strategy for antagonizing ovarian cancer cell motility and invasiveness

Authors - Yanchun Zhang
View Article