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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using GAGE7 Polyclonal antibody (12945-1-AP)

    WB analysis using 12945-1-AP (same clone as 12945-1-PBS)

    LNCaP cells were subjected to SDS PAGE followed by western blot with 12945-1-AP (GAGE7 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12945-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of LNCaP cells using GAGE7 Polyclonal antibody (12945-1-AP)

    WB analysis of LNCaP using 12945-1-AP (same clone as 12945-1-PBS)

    WB result of GAGE7 antibody (12945-1-AP; 1:600; incubated at room temperature for 1.5 hours) with sh-Control and sh-GAGE7 transfected LNCaP cells. This data was developed using the same antibody clone with 12945-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using GAGE7 Polyclonal antibody (12945-1-AP)

    WB analysis using 12945-1-AP (same clone as 12945-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 12945-1-AP (GAGE7 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12945-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human gliomas tissue using GAGE7 Polyclonal antibody (12945-1-AP)

    IHC staining of human gliomas using 12945-1-AP (same clone as 12945-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 12945-1-AP (GAGE7 Antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 12945-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human gliomas tissue using GAGE7 Polyclonal antibody (12945-1-AP)

    IHC staining of human gliomas using 12945-1-AP (same clone as 12945-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 12945-1-AP (GAGE7 Antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 12945-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human prostate cancer tissue using GAGE7 Polyclonal antibody (12945-1-AP)

    IHC staining of human prostate cancer using 12945-1-AP (same clone as 12945-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 12945-1-AP (GAGE7 Antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 12945-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human prostate cancer tissue using GAGE7 Polyclonal antibody (12945-1-AP)

    IHC staining of human prostate cancer using 12945-1-AP (same clone as 12945-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 12945-1-AP (GAGE7 Antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 12945-1-PBS in a different storage buffer formulation.

GAGE7 Polyclonal antibody, PBS Only

GAGE7 Polyclonal Antibody for WB, IHC, Indirect ELISA
Cat No. 12945-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, Indirect ELISA

Synonyms

AL4, Cancer/testis antigen 4.7, CT4.7, G antigen 12F, G antigen 7

Formulation:  PBS Only
PBS, Azide, Glycerol
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

12945-1-PBS targets GAGE7 in WB, IHC, Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag4018

Product name: Recombinant human GAGE7 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-117 aa of BC031628

Sequence: MSWRGRSTYYWPRPRRYVQPPEMIGPMRPEQFSDEVEPATPEEGEPATQRQDPAAAQEGEDEGASAGQGPKPEAHSQEQGHPQTGCECEDGPDGQEMDPPNPEEVKTPEEGEKQSQC

相同性解析による交差性が予測される生物種
Full Name G antigen 7
Calculated molecular weight 13 kDa
Observed molecular weight26 kDa
GenBank accession numberBC031628
Gene Symbol GAGE7
Gene ID (NCBI) 2579
RRIDAB_2877895
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDO76087
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

GAGE7 (G Antigen 7), also known as CT4.7 or GAGE-7, is a cancer-testis antigen belonging to the GAGE family. It is predominantly expressed in germ cells of the testis and various tumors, including melanoma, lung, and breast cancers, while remaining silent in normal adult tissues (PMID: 10397259). GAGE7 is an intrinsically disordered protein (IDP) of 117 amino acids with a theoretical molecular weight of ~13 kDa; however, it exhibits an apparent molecular weight of ~26 kDa in SDS-PAGE due to its abnormal electrophoretic mobility (PMID: 23029259).

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