Validation Data Gallery

Filter: AllWBIPIHC

WB analysis using 16260-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 16260-1-AP (GADD45GIP1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of mouse heart using 16260-1-AP
mouse heart tissue were subjected to SDS PAGE followed by western blot with 16260-1-AP (GADD45GIP1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.
WB analysis of HEK-293 using 16260-1-AP
HEK-293 cells were subjected to SDS PAGE followed by western blot with 16260-1-AP (GADD45GIP1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of SKOV-3 using 16260-1-AP
SKOV-3 cells were subjected to SDS PAGE followed by western blot with 16260-1-AP (GADD45GIP1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of NIH/3T3 using 16260-1-AP
NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 16260-1-AP (GADD45GIP1 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.
WB analysis of mouse heart using 16260-1-AP
mouse heart tissue were subjected to SDS PAGE followed by western blot with 16260-1-AP (GADD45GIP1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
IP experiment of mouse heart using 16260-1-AP
IP result of anti-GADD45GIP1 (IP:16260-1-AP, 4ug; Detection:16260-1-AP 1:400) with mouse heart tissue lysate 4000ug.
IHC staining of human ovary tumor using 16260-1-AP
Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 16260-1-AP (GADD45GIP1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of mouse brain using 16260-1-AP
Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 16260-1-AP (GADD45GIP1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human ovary tumor using 16260-1-AP
Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 16260-1-AP (GADD45GIP1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

GADD45GIP1 Polyclonal antibody

GADD45GIP1 Polyclonal Antibody for WB, IHC, IP, ELISA

Cat No. 16260-1-AP

Publications(7)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (1)

Applications

WB, IHC, IF, IP, ELISA

Synonyms

Growth arrest and DNA damage-inducible proteins-interacting protein 1, CRIF1, CR6-interacting factor 1, CKII beta-associating protein, CKBBP2

Formulation: PBS, Azide, Glycerol

PBS, Azide, Glycerol

Conjugate: Unconjugated

Unconjugated

Size/Concentration:

-/ -

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Overview

Tested Applications
Product Information
Publications (7)
Protocols

データシート［PDF］を印刷

MSDS

Tested Applications

Positive WB detected in	HeLa cells, NIH/3T3 cells, mouse heart tissue, HEK-293 cells, SKOV-3 cells
Positive IP detected in	mouse heart tissue
Positive IHC detected in	human ovary tumor tissue, mouse brain tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:500-1:2000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)	IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WB	See 5 publications below
IHC	See 2 publications below
IF	See 1 publications below

Product Information

16260-1-AP targets GADD45GIP1 in WB, IHC, IF, IP, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity	human, mouse
Cited Reactivity	human, mouse, rat
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag9299 Product name: Recombinant human GADD45GIP1 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-222 aa of BC013027 Sequence: AASVRQARSLLGVAATLAPGSRGYRARPPPRRRPGPRWPDPEDLLTPRWQLGPRYAAKQFARYGAASGVVPGSLWPSPEQLRELEAEEREWYPSLATMQESLRVKQLAEEQKRREREQHIAECMAKMPQMIVNWQQQQRENWEKAQADKERRARLQAEAQELLGYQVDPRSARFQELLQDLEKKERKRLKEEKQKRKKEARAAALAAAVAQDPAASGAPSS 相同性解析による交差性が予測される生物種
Full Name	growth arrest and DNA-damage-inducible, gamma interacting protein 1
Calculated molecular weight	222 aa, 25 kDa
Observed molecular weight	25-28 kDa
GenBank accession number	BC013027
Gene Symbol	GADD45GIP1
Gene ID (NCBI)	90480
RRID	AB_2108229
Conjugate	Unconjugated
Form
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	Q8TAE8
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Protocols

Product Specific Protocols
IHC protocol for GADD45GIP1 antibody 16260-1-AP	Download protocol
IP protocol for GADD45GIP1 antibody 16260-1-AP	Download protocol
WB protocol for GADD45GIP1 antibody 16260-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
mouse	WB,IF	Science T cell metabolism. The protein LEM promotes CD8⁺ T cell immunity through effects on mitochondrial respiration. Authors - Isobel Okoye View Article
		Cell Mol Life Sci GTPBP8 is required for mitoribosomal biogenesis and mitochondrial translation Authors - Liang Wang View Article
human	IHC	Oncotarget CR6-interacting factor 1 inhibits invasiveness by suppressing TGF-β-mediated epithelial-mesenchymal transition in hepatocellular carcinoma. Authors - Runzhou Zhuang View Article
rat	WB	J Mol Med (Berl) Effect of DJ-1 on the neuroprotection of astrocytes subjected to cerebral ischemia/reperfusion injury. Authors - Li Peng View Article
mouse	WB	Burns Trauma Cardiac proteomics reveals the potential mechanism of microtubule associated protein 4 phosphorylation-induced mitochondrial dysfunction. Authors - Lingfei Li View Article
mouse	WB,IHC	Int J Mol Sci Nmnat1 Deficiency Causes Mitoribosome Excess in Diabetic Nephropathy Mediated by Transcriptional Repressor HIC1 Authors - Kazuhiro Hasegawa View Article

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Validation Data Gallery

WB analysis using 16260-1-AP

WB analysis of mouse heart using 16260-1-AP

WB analysis of HEK-293 using 16260-1-AP

WB analysis of SKOV-3 using 16260-1-AP

WB analysis of NIH/3T3 using 16260-1-AP

WB analysis of mouse heart using 16260-1-AP

IP experiment of mouse heart using 16260-1-AP

IHC staining of human ovary tumor using 16260-1-AP

IHC staining of mouse brain using 16260-1-AP

IHC staining of human ovary tumor using 16260-1-AP

GADD45GIP1 Polyclonal antibody

GADD45GIP1 Polyclonal Antibody for WB, IHC, IP, ELISA

-/ -

Tested Applications

Recommended dilution

Published Applications

Product Information

Protocols

Publications

T cell metabolism. The protein LEM promotes CD8⁺ T cell immunity through effects on mitochondrial respiration.

GTPBP8 is required for mitoribosomal biogenesis and mitochondrial translation

CR6-interacting factor 1 inhibits invasiveness by suppressing TGF-β-mediated epithelial-mesenchymal transition in hepatocellular carcinoma.

Effect of DJ-1 on the neuroprotection of astrocytes subjected to cerebral ischemia/reperfusion injury.

Cardiac proteomics reveals the potential mechanism of microtubule associated protein 4 phosphorylation-induced mitochondrial dysfunction.

Nmnat1 Deficiency Causes Mitoribosome Excess in Diabetic Nephropathy Mediated by Transcriptional Repressor HIC1