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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using GAD1 Polyclonal antibody (10408-1-AP)

    WB analysis using 10408-1-AP (same clone as 10408-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 10408-1-AP (GAD1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10408-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using GAD1 Polyclonal antibody (10408-1-AP)

    WB analysis using 10408-1-AP (same clone as 10408-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 10408-1-AP (GAD1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10408-1-PBS in a different storage buffer formulation.

  • Immunoprecipitation (IP) experiment of mouse brain tissue using GAD1 Polyclonal antibody (10408-1-AP)

    IP experiment of mouse brain using 10408-1-AP (same clone as 10408-1-PBS)

    IP result of anti-GAD1 (IP:10408-1-AP, 4ug; Detection:10408-1-AP 1:500) with mouse brain tissue lysate 1920 ug. This data was developed using the same antibody clone with 10408-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse brain tissue using GAD1 Polyclonal antibody (10408-1-AP)

    IHC staining of mouse brain using 10408-1-AP (same clone as 10408-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 10408-1-AP (GAD1 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 10408-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse brain tissue using GAD1 Polyclonal antibody (10408-1-AP)

    IHC staining of mouse brain using 10408-1-AP (same clone as 10408-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 10408-1-AP (GAD1 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 10408-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse brain tissue using GAD1 Polyclonal antibody (10408-1-AP)

    IHC staining of mouse brain using 10408-1-AP (same clone as 10408-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 10408-1-AP (GAD1 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 10408-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse brain tissue using GAD1 Polyclonal antibody (10408-1-AP)

    IHC staining of mouse brain using 10408-1-AP (same clone as 10408-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 10408-1-AP (GAD1 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 10408-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse cerebellum tissue using GAD1 Polyclonal antibody (10408-1-AP)

    IHC staining of mouse cerebellum using 10408-1-AP (same clone as 10408-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 10408-1-AP (GAD1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 10408-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse cerebellum tissue using GAD1 Polyclonal antibody (10408-1-AP)

    IHC staining of mouse cerebellum using 10408-1-AP (same clone as 10408-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 10408-1-AP (GAD1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 10408-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of mouse brain tissue using GAD1 Polyclonal antibody (10408-1-AP)

    IF Staining of mouse brain using 10408-1-AP (same clone as 10408-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed frozen OCT-embedded mouse brain tissue using GAD1 antibody (10408-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 10408-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO Validated

GAD1 Polyclonal antibody, PBS Only

GAD1 Polyclonal Antibody for WB, IHC, IF-Fro, IP, ELISA
Cat No. 10408-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF-Fro, IP, ELISA

Synonyms

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

10408-1-PBS targets GAD1 in WB, IHC, IF-Fro, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag0666

Product name: Recombinant human GAD1 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-224 aa of BC002815

Sequence: MASSTPSSSATSSNAGADPNTTNLRPTTYDTWCGVAHGCTRKLGLKICGFLQRTNSLEEKSRLVSAFKERQSSKNLLSCENSDRDARFRRTETDFSNLFARDLLPAKNGEEQTVQFLLEVVDILLNYVRKTFDRSTKVLDFHHPHQLLEGMEGFNLELSDHPESLEQILVDCRDTLKYGVRTGHPRFFNQLSTGLDIIGLAGEWLTSTANTNMPSDMRECWLLR

相同性解析による交差性が予測される生物種
Full Name glutamate decarboxylase 1 (brain, 67kDa)
Calculated molecular weight 67 kDa
Observed molecular weight 67 kDa
GenBank accession numberBC002815
Gene Symbol GAD1
Gene ID (NCBI) 2571
RRIDAB_2107733
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDQ99259
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

The GAD1(glutamate decarboxylase 1) gene encodes 67 kDa glutamic acid decarboxylase isoform (GAD67), the rate-limiting enzyme responsible for γ-aminobutyric acid (GABA) biosynthesis from glutamatic acid and the major GAD isoform in the human brain for early brain development (PMID:21302352). It is also potentially involved in variety of skin activities. It belongs to the group II decarboxylase family.

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