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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using GABRA5 Polyclonal antibody (27929-1-AP)

    WB analysis using 27929-1-AP (same clone as 27929-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 27929-1-AP (GABRA5 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 27929-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse brain tissue using GABRA5 Polyclonal antibody (27929-1-AP)

    IHC staining of mouse brain using 27929-1-AP (same clone as 27929-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 27929-1-AP (GABRA5 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 27929-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse brain tissue using GABRA5 Polyclonal antibody (27929-1-AP)

    IHC staining of mouse brain using 27929-1-AP (same clone as 27929-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 27929-1-AP (GABRA5 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 27929-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of mouse brain tissue using GABRA5 Polyclonal antibody (27929-1-AP)

    IF Staining of mouse brain using 27929-1-AP (same clone as 27929-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed frozen OCT-embedded mouse brain tissue using GABRA5 antibody (27929-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 27929-1-PBS in a different storage buffer formulation.

GABRA5 Polyclonal antibody, PBS Only

GABRA5 Polyclonal Antibody for WB, IHC, IF-Fro, Indirect ELISA
Cat No. 27929-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF-Fro, Indirect ELISA

Synonyms

GABA A receptor alpha 5, GABA(A) receptor subunit alpha-5, GABAA receptor alpha 5, GABAAR subunit alpha-5, Gamma-aminobutyric acid receptor subunit alpha-5

Formulation:  PBS Only
PBS, Azide, Glycerol
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

27929-1-PBS targets GABRA5 in WB, IHC, IF-Fro, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag27376

Product name: Recombinant human GABRA5 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 36-222 aa of BC113422

Sequence: SSVKDETNDNITIFTRILDGLLDGYDNRLRPGLGERITQVRTDIYVTSFGPVSDTEMEYTIDVFFRQSWKDERLRFKGPMQRLPLNNLLASKIWTPDTFFHNGKKSIAHNMTTPNKLLRLEDDGTLLYTMRLTISAECPMQLEDFPMDAHACPLKFGSYAYPNSEVVYVWTNGSTKSVVVAEDGSRL

相同性解析による交差性が予測される生物種
Full Name gamma-aminobutyric acid (GABA) A receptor, alpha 5
Calculated molecular weight 462 aa, 52 kDa
Observed molecular weight52 kDa
GenBank accession numberBC113422
Gene Symbol GABRA5
Gene ID (NCBI) 2558
RRIDAB_2923580
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDP31644
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.
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