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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

GABRA4 Recombinant antibody

GABRA4 Recombinant Antibody for FC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human

Applications

FC, ELISA

Conjugate

Unconjugated

CloneNo.

230302A4

Cat no : 83030-4-RR

Synonyms

GABRA4

Validation Data Gallery

Applications:
  • FC experiment of SH-SY5Y using 83030-4-RR

    FC experiment of SH-SY5Y using 83030-4-RR

    1x10^6 SH-SY5Y cells were intracellularly stained with 0.25 ug Anti-Human A4' target='_blank'>GABRA4 (83030-4-RR, Clone:230302A4) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • FC experiment of PC-3 using 83030-4-RR

    FC experiment of PC-3 using 83030-4-RR

    1x10^6 PC-3 cells were intracellularly stained with 0.25 ug Anti-Human A4' target='_blank'>GABRA4 (83030-4-RR, Clone:230302A4) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

Tested Applications

Positive FC detected inPC-3 cells, SH-SY5Y cells

Recommended dilution

ApplicationDilution
Flow Cytometry (FC)FC : 0.25 ug per 10^6 cells in a 100 µl suspension
Sample-dependent, check data in validation data gallery

Product Information

83030-4-RR targets GABRA4 in FC, ELISA applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen GABRA4 fusion protein Ag3600 相同性解析による交差性が予測される生物種
Full Name gamma-aminobutyric acid (GABA) A receptor, alpha 4
Calculated molecular weight 554 aa, 62 kDa
Observed molecular weight 62-70 kDa
GenBank accession numberBC035055
Gene symbol GABRA4
Gene ID (NCBI) 2557
Conjugate Unconjugated
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Gamma-aminobutyric acid (GABA) is the major inhibitory neurotransmitter in the mammalian brain where it acts at GABA-A receptors, which are ligand-gated chloride channels. Chloride conductance of these channels can be modulated by agents such as benzodiazepines that bind to the GABA-A receptor. GABRA4, encoding the extrasynaptically located α4‐subunit of the GABAA receptor, is involved in the etiology of autism. (PMID: 32028044, 32033586)

Protocols

Product Specific Protocols
FC protocol for GABRA4 antibody 83030-4-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols