Validation Data Gallery
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Tested Applications
| Positive WB detected in | mouse brain tissue, rat brain tissue |
| Positive IP detected in | mouse brain tissue |
| Positive IHC detected in | rat brain tissue, mouse brain tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:16000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 1 publications below |
| WB | See 32 publications below |
| IHC | See 2 publications below |
| IF | See 10 publications below |
| IP | See 1 publications below |
| FC | See 1 publications below |
Product Information
12410-1-AP targets GABRA1 in WB, IHC, IF, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, rat, zebrafish |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag3117 Product name: Recombinant human GABRA1 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 21-280 aa of BC030696 Sequence: LTGRSYGQPSLQDELKDNTTVFTRILDRLLDGYDNRLRPGLGERVTEVKTDIFVTSFGPVSDHDMEYTIDVFFRQSWKDERLKFKGPMTVLRLNNLMASKIWTPDTFFHNGKKSVAHNMTMPNKLLRITEDGTLLYTMRLTVRAECPMHLEDFPMDAHACPLKFGSYAYTRAEVVYEWTREPARSVVVAEDGSRLNQYDLLGQTVDSGIVQSSTGEYVVMTTHFHLKRKIGYFVIQTYLPCIMTVILSQVSFWLNRESVP 相同性解析による交差性が予測される生物種 |
| Full Name | gamma-aminobutyric acid (GABA) A receptor, alpha 1 |
| Calculated molecular weight | 456 aa, 52 kDa |
| Observed molecular weight | 52 kDa |
| GenBank accession number | BC030696 |
| Gene Symbol | GABRA1 |
| Gene ID (NCBI) | 2554 |
| RRID | AB_2108692 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P14867 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for GABRA1 antibody 12410-1-AP | Download protocol |
| IP protocol for GABRA1 antibody 12410-1-AP | Download protocol |
| WB protocol for GABRA1 antibody 12410-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Nat Biotechnol Magnify is a universal molecular anchoring strategy for expansion microscopy | ||
Adv Mater Noninvasive Optogenetics Realized by iPSC-Derived Tentacled Carrier in Alzheimer's Disease Treatment | ||
Neurol Neuroimmunol Neuroinflamm MRI Characteristics of Autoimmune Encephalitis With Autoantibodies to GABAA Receptor: A Case Series. | ||
Mol Ther Nucleic Acids Modulation of miR-181 influences dopaminergic neuronal degeneration in a mouse model of Parkinson's disease. | ||
Int J Mol Sci The Effect of TGF-β1 Reduced Functionality on the Expression of Selected Synaptic Proteins and Electrophysiological Parameters: Implications of Changes Observed in Acute Hepatic Encephalopathy. |
