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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

GABPB2 Polyclonal antibody

GABPB2 Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, ELISA

Conjugate

Unconjugated

Cat no : 20870-1-AP

Synonyms

GABP subunit beta 2, GABPB 2, GABPB2

Validation Data Gallery

Applications:
  • WB analysis of Jurkat using 20870-1-AP

    WB analysis of Jurkat using 20870-1-AP

    Jurkat cells were subjected to SDS PAGE followed by western blot with 20870-1-AP (GABPB2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • WB analysis of HeLa using 20870-1-AP

    WB analysis of HeLa using 20870-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 20870-1-AP (GABPB2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • WB analysis of HEK-293 using 20870-1-AP

    WB analysis of HEK-293 using 20870-1-AP

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 20870-1-AP (GABPB2 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • WB analysis of HeLa using 20870-1-AP

    WB analysis of HeLa using 20870-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 20870-1-AP (GABPB2 antibody) at dilution of 1:100 incubated at room temperature for 1.5 hours.

  • WB analysis of A375 using 20870-1-AP

    WB analysis of A375 using 20870-1-AP

    A375 cells were subjected to SDS PAGE followed by western blot with 20870-1-AP (GABPB2 antibody) at dilution of 1:100 incubated at room temperature for 1.5 hours.

Tested Applications

Positive WB detected inJurkat cells, A375 cells, HEK-293 cells, HeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Sample-dependent, check data in validation data gallery

Product Information

The immunogen of 20870-1-AP is GABPB2 Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse, rat
Cited Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen GABPB2 fusion protein Ag14933 相同性解析による交差性が予測される生物種
Full Name GA binding protein transcription factor, beta subunit 2
Calculated molecular weight 448 aa, 49 kDa
Observed molecular weight 49 kDa
GenBank accession numberBC027033
Gene symbol GABPB2
Gene ID (NCBI) 126626
RRIDAB_10734321
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for GABPB2 antibody 20870-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanIP

Mol Cell

Argininosuccinate lyase drives activation of mutant TERT promoter in glioblastomas

Authors - Zhumei Shi
  • KD Validated
View Article