Validation Data Gallery
Tested Applications
| Positive WB detected in | HEK-293 cells, HeLa cells, SH-SY5Y cells, RAW 264.7cells, NIH/3T3 cells, mouse brain tissue, rat brain tissue |
| Positive IF/ICC detected in | Chloroquine treated HepG2 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:250-1:1000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
86165-3-RR targets GABARAP in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Recombinant |
| Type | Antibody |
| Immunogen |
Peptide 相同性解析による交差性が予測される生物種 |
| Full Name | GABA(A) receptor-associated protein |
| Calculated molecular weight | 14 kDa |
| Observed molecular weight | 14-18 kDa |
| GenBank accession number | BC106748 |
| Gene Symbol | GABARAP |
| Gene ID (NCBI) | 11337 |
| RRID | AB_3744675 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | O95166 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
GABARAP (Gamma-aminobutyric acid type A receptor-associated protein), was initially identified as GABAA receptor binding protein and has shown to be implicated in intracellular trafficking of GABAA receptors. Through interacting with a broad rang of proteins, GABARAP has been demonstrated as a multifunctional protein implicated in intracellular vesicle trafficking, apoptosis, and autophagy. The protein level of lipidated form of GABARAP (GABARAP-II) is upregulated during the autophagic process, which makes it as an autophagic marker. This antibody detected endogenous GABARAP protein in lysates from various tissues. It may cross-react with GABARAPL1 due to the high identity of amino acid sequence.
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for GABARAP antibody 86165-3-RR | Download protocol |
| WB protocol for GABARAP antibody 86165-3-RR | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |