Validation Data Gallery
Tested Applications
| Positive WB detected in | HeLa cells, mouse brain tissue, SH-SY5Y cells, Chloroquine treated HEK-293 cells |
| Positive IHC detected in | human ovary cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:3000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
22439-1-AP targets GABARAP in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.
| Tested Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag18209 Product name: Recombinant human GABARAP protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-117 aa of BC106749 Sequence: MKFVYKEEHPFEKRRSEGEKIRKKYPDRVPVIVEKAPKARIGDLDKKKYLVPSDLTVGQFYFLIRKRIHLRAEDALFFFVNNVIPPTSATMGQLYQEHHEEDFFLYIAYSDESVYGL 相同性解析による交差性が予測される生物種 |
| Full Name | GABA(A) receptor-associated protein |
| Calculated molecular weight | 117 aa, 14 kDa |
| Observed molecular weight | 19-21 kDa |
| GenBank accession number | BC106749 |
| Gene Symbol | GABARAP |
| Gene ID (NCBI) | 11337 |
| RRID | AB_2879106 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | O95166 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for GABARAP antibody 22439-1-AP | Download protocol |
| WB protocol for GABARAP antibody 22439-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |




