Various lysates were subjected to SDS PAGE followed by western blot with 18723-1-AP (GABARAP antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

A549 cells were subjected to SDS PAGE followed by western blot with 18723-1-AP (GABARAP antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

mouse brain tissue were subjected to SDS PAGE followed by western blot with 18723-1-AP (GABARAP antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

human placenta tissue were subjected to SDS PAGE followed by western blot with 18723-1-AP (GABARAP antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

mouse pancreas tissue were subjected to SDS PAGE followed by western blot with 18723-1-AP (GABARAP antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

human heart tissue were subjected to SDS PAGE followed by western blot with 18723-1-AP (GABARAP antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 18723-1-AP (GABARAP antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

Various lysates were subjected to SDS PAGE followed by western blot with 18723-1-AP (GABARAP antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

IP result of anti-GABARAP (IP:18723-1-AP, 4ug; Detection:18723-1-AP 1:2000) with HeLa cells lysate 1200 ug.

Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 18723-1-AP (GABARAP antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 18723-1-AP (GABARAP antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Immunofluorescent analysis of (-20°C Ethanol) fixed Chloroquine treated HepG2 cells and untreated HepG2 cells using GABARAP antibody (18723-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Immunofluorescent analysis of (-20℃ Ethanol) fixed Starvation treated HepG2 cells and untreated HepG2 cells using 18723-1-AP (GABARAP antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

1X10^6 HepG2 cells were intracellularly stained with 0.4 ug Anti-Human GABARAP (18723-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Isotype Control. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer.