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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using G6PC Polyclonal antibody (29084-1-AP)

    WB analysis using 29084-1-AP (same clone as 29084-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 29084-1-AP (G6PC antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 29084-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of mouse liver tissue using G6PC Polyclonal antibody (29084-1-AP)

    IF Staining of mouse liver using 29084-1-AP (same clone as 29084-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse liver tissue using G6PC antibody (29084-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 29084-1-PBS in a different storage buffer formulation.

G6PC Polyclonal antibody, PBS Only

G6PC Polyclonal Antibody for WB, IF-P, Indirect ELISA
Cat No. 29084-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IF-P, Indirect ELISA

Synonyms

G6PC1, EC:3.1.3.9, G6Pase, G-6-Pase, G6Pase-alpha

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

29084-1-PBS targets G6PC in WB, IF-P, Indirect ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag30614

Product name: Recombinant human G6PC protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 72-121 aa of BC130478

Sequence: NLVFKWILFGQRPYWWVLDTDYYSNTSVPLIKQFPVTCETGPGSPSGHAM

相同性解析による交差性が予測される生物種
Full Name glucose-6-phosphatase, catalytic subunit
Calculated molecular weight 357 aa, 40 kDa
Observed molecular weight 40 kDa
GenBank accession numberBC130478
Gene Symbol G6PC
Gene ID (NCBI) 2538
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDP35575
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

Glucose-6-phosphatase-α (G6PC) is a key enzyme in glucose homeostasis that catalyzes the hydrolysis of glucose-6-phosphate to glucose and phosphate in the terminal step of gluconeogenesis and glycogenolysis. G6PC activity is restricted to the liver , the kidney cortex and the small intestine and confers on these three organs the capacity to release glucose into the systemic circulation (PMID: 21983240).The encoded enzyme is anchored to the ER by nine transmembrane helices with the amino (N)-terminus in the lumen and the carboxyl (C)-terminus in the cytoplasm (PMID: 15542400).