Validation Data Gallery
Tested Applications
Recommended dilution
| Application | Dilution |
|---|---|
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Product Information
34058-1-PBS targets G2E3 in IHC, IP, Indirect ELISA applications and shows reactivity with human, mouse samples.
| Tested Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag40477 Product name: Recombinant human G2E3 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 231-340 aa of BC000973 Sequence: ERCDVRRCRCKEGRDYNAPDSKWEIKRCQCCGSSGTHLACSSLRSWEQNWECLECRGIIYNSGEFQKAKKHVLPNSNNVGITDCLLEESSPKLPRQSPGSQSKDLLRQGS 相同性解析による交差性が予測される生物種 |
| Full Name | G2/M-phase specific E3 ubiquitin ligase |
| Calculated molecular weight | 706 aa, 81 kDa |
| Observed molecular weight | 80 kDa |
| GenBank accession number | BC000973 |
| Gene Symbol | G2E3 |
| Gene ID (NCBI) | 55632 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Antigen affinity Purification |
| UNIPROT ID | Q7L622 |
| Storage Buffer | PBS only{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -80°C. |
Background Information
G2/M-phase specific E3 ubiquitin protein ligase (G2E3) is a cell cycle-regulated E3 ubiquitin ligase that plays a critical role in controlling the G2/M transition. It functions by targeting specific substrates for proteasomal degradation during late G2 and M phases, thereby ensuring proper cell cycle progression. G2E3 contains a RING finger domain, characteristic of E3 ubiquitin ligases, and its expression is tightly regulated in a cell cycle-dependent manner, peaking during G2/M phases. Research has implicated G2E3 in various cellular processes, including DNA damage response and mitotic regulation (PMID: 17239372; PMID: 18511420; PMID: 41068090).