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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using Frizzled 5 Polyclonal antibody (21519-1-AP)

    WB analysis using 21519-1-AP (same clone as 21519-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 21519-1-AP (Frizzled 5 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 21519-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using Frizzled 5 Polyclonal antibody (21519-1-AP)

    WB analysis using 21519-1-AP (same clone as 21519-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 21519-1-AP (Frizzled 5 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 21519-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human stomach cancer tissue using Frizzled 5 Polyclonal antibody (21519-1-AP)

    IHC staining of human stomach cancer using 21519-1-AP (same clone as 21519-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 21519-1-AP (Frizzled 5 antibody) at dilution of 1:500 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 21519-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human prostate cancer tissue using Frizzled 5 Polyclonal antibody (21519-1-AP)

    IHC staining of human prostate cancer using 21519-1-AP (same clone as 21519-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human prostate cancer using 21519-1-AP (FZD5 antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 21519-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human prostate cancer tissue using Frizzled 5 Polyclonal antibody (21519-1-AP)

    IHC staining of human prostate cancer using 21519-1-AP (same clone as 21519-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human prostate cancer using 21519-1-AP (FZD5 antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 21519-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using Frizzled 5 Polyclonal antibody (21519-1-AP)

    IF Staining of HepG2 using 21519-1-AP (same clone as 21519-1-PBS)

    Immunofluorescent analysis of (-20°C Methanol) fixed HepG2 cells using Frizzled 5 antibody (21519-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 21519-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using Frizzled 5 Polyclonal antibody (21519-1-AP)

    IF Staining of HepG2 using 21519-1-AP (same clone as 21519-1-PBS)

    Immunofluorescent analysis of (-20°C Methanol) fixed HepG2 cells using Frizzled 5 antibody (21519-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 21519-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO Validated

Frizzled 5 Polyclonal antibody, PBS Only

Frizzled 5 Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 21519-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, Indirect ELISA

Synonyms

FZD5, FzE5, Fz-5, Fz 5, Frizzled-5

Formulation:  PBS Only
PBS, Azide, Glycerol
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

21519-1-PBS targets Frizzled 5 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag16082

Product name: Recombinant human FZD5 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 524-585 aa of BC114346

Sequence: GKTVESWRRFTSRCCCRPRRGHKSGGAMAAGDYPEASAALTGRTGPPGPAATYHKQVSLSHV

相同性解析による交差性が予測される生物種
Full Name frizzled homolog 5 (Drosophila)
Calculated molecular weight 585 aa, 65 kDa
Observed molecular weight 65-70 kDa
GenBank accession numberBC114346
Gene Symbol FZD5
Gene ID (NCBI) 7855
RRIDAB_2878877
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDQ13467
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

Fzd5, is the receptor for the Wnt5A ligand. Fzd5 modulates cell fate and cell behavior during vertebrate development. It regulates hematopoiesis by the antagonism of the canonical Wnt pathway, resulting in a pool of quiescent hematopoietic stem cells, and regulates hematopoietic stem cell function in a paracrine fashion through several distinct mechanisms including modulating canonical Wnt signaling. It acts through noncanonical Wnt signaling to promote angiogenesis. It is also involved in control cell orientation, polarity, and directional movement in response to positional cues from chemokine gradients.

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