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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using Follistatin Polyclonal antibody (27350-1-AP)

    WB analysis using 27350-1-AP (same clone as 27350-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 27350-1-AP (Follistatin antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 27350-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using Follistatin Polyclonal antibody (27350-1-AP)

    WB analysis using 27350-1-AP (same clone as 27350-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 27350-1-AP (Follistatin antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 27350-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using Follistatin Polyclonal antibody (27350-1-AP)

    IF Staining of HeLa using 27350-1-AP (same clone as 27350-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using Follistatin antibody (27350-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 27350-1-PBS in a different storage buffer formulation.

Follistatin Polyclonal antibody, PBS Only

Follistatin Polyclonal Antibody for WB, IF/ICC, Indirect ELISA
Cat No. 27350-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, Indirect ELISA

Synonyms

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

27350-1-PBS targets Follistatin in WB, IF/ICC, Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag26401

Product name: Recombinant human FST protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 135-334 aa of BC004107

Sequence: DGKTYRNECALLKARCKEQPELEVQYQGRCKKTCRDVFCPGSSTCVVDQTNNAYCVTCNRICPEPASSEQYLCGNDGVTYSSACHLRKATCLLGRSIGLAYEGKCIKAKSCEDIQCTGGKKCLWDFKVGRGRCSLCDELCPDSKSDEPVCASDNATYASECAMKEAACSSGVLLEVKHSGSCNSISEDTEEEEEDEDQDY

相同性解析による交差性が予測される生物種
Full Name follistatin
Calculated molecular weight 33 aa, 7 kDa
Observed molecular weight 35 kDa
GenBank accession numberBC004107
Gene Symbol Follistatin
Gene ID (NCBI) 10468
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDP19883
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

Follistatin (FST) is a member of the tissue growth factor β family and is a secreted glycoprotein that antagonizes many members of the family, including activin A, growth differentiation factor11 , and myostatin. It binds activin A with high affinity and whose expression can be induced by activin A and several other pro-inflammatory cytokines. Activin A-follistatin complexes are biologically inactive and bind to cell surface heparan sulphate-containing proteoglycans for internalisation and degradation. It has also been found to play a significant role in the management of skeletal muscle size and mass. It also has important roles in early embryonic development, differentiation of ovarian granulosa cells, liver fibrosis and polycystic ovarian syndrome.

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