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Validation Data Gallery

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  • Western Blot (WB) analysis of A549 cells using Flotillin 2 Recombinant monoclonal antibody (88149-1-RR)

    WB analysis of A549 using 88149-1-RR

    WB result of Flotillin 2 antibody (88149-1-RR; 1:3000; incubated at room temperature for 1.5 hours) with sh-Control and sh-Flotillin 2 transfected A549 cells.

  • Western Blot (WB) analysis of various lysates using Flotillin 2 Recombinant monoclonal antibody (88149-1-RR)

    WB analysis using 88149-1-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 88149-1-RR (Flotillin 2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using Flotillin 2 Recombinant monoclonal antibody (88149-1-RR)

    IHC staining of human colon cancer using 88149-1-RR

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 88149-1-RR (Flotillin 2 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using Flotillin 2 Recombinant monoclonal antibody (88149-1-RR)

    IHC staining of human colon cancer using 88149-1-RR

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 88149-1-RR (Flotillin 2 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using Flotillin 2 Recombinant monoclonal antibody (88149-1-RR)

    IF Staining of HeLa using 88149-1-RR

    Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using Flotillin 2 antibody (88149-1-RR, Clone: 260368C2 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

  • KD/KO Validated

Flotillin 2 Recombinant monoclonal antibody

Flotillin 2 Uni-rAb® Recombinant Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 88149-1-RR
Clone No.260368C2

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Synonyms

FLOT2, Flotillin-2, ECS 1, ECS1, Epidermal surface antigen

Formulation:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

ご購入について

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約2万点のプロテインテック製品をコスモバイオ社物流センター（国内）に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。

保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inHeLa cells, A549 cells, A375 cells, A431 cells, HEK-293 cells, NIH/3T3 cells, PC-12 cells
Positive IHC detected inhuman colon cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

88149-1-RR targets Flotillin 2 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen

CatNo: Ag28308

Product name: Recombinant human Flotillin 2 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 1-379 aa of BC017292

Sequence: MTLQPRCEDVETAEGVALTVTGVAQVKIMTEKELLAVACEQFLGKNVQDIKNVVLQTLEGHLRSILGTLTVEQIYQDRDQFAKLVREVAAPDVGRMGIEILSFTIKDVYDKVDYLSSLGKTQTAVVQRDADIGVAEAERDAGIREAECKKEMLDVKFMADTKIADSKRAFELQKSAFSEEVNIKTAEAQLAYELQGAREQQKIRQEEIEIEVVQRKKQIAVEAQEILRTDKELIATVRRPAEAEAHRIQQIAEGEKVKQVLLAQAEAEKIRKIGEAEAAVIEAMGKAEAERMKLKAEAYQKYGDAAKMALVLEALPQIAAKIAAPLTKVDEIVVLSGDNSKVTSEVNRLLAELPASVHALTGVDLSKIPLIKKATGVQV

相同性解析による交差性が予測される生物種
Full Name flotillin 2
Calculated molecular weight 379 aa, 42 kDa
Observed molecular weight 48 kDa
GenBank accession numberBC017292
Gene Symbol Flotillin 2
Gene ID (NCBI) 2319
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein A purification
UNIPROT IDQ14254
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Flotillins are membrane-associated proteins that contain two homologous isoforms, flotillin 1 (FLOT1) and flotillin 2 (FLOT2). They are highly conserved and widely expressed proteins that commonly used as markers of lipid rafts. Flotillins are involved in various signaling pathways, cell adhesion, membrane trafficking and axonal growth.

Protocols

Product Specific Protocols
IF protocol for Flotillin 2 antibody 88149-1-RRDownload protocol
IHC protocol for Flotillin 2 antibody 88149-1-RRDownload protocol
WB protocol for Flotillin 2 antibody 88149-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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