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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using Flotillin 1 Monoclonal antibody (67968-1-Ig)

    WB analysis using 67968-1-Ig (same clone as 67968-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67968-1-Ig (Flotillin 1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67968-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human ovary tumor tissue using Flotillin 1 Monoclonal antibody (67968-1-Ig)

    IHC staining of human ovary tumor using 67968-1-Ig (same clone as 67968-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 67968-1-Ig (Flotillin 1 antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67968-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human stomach cancer tissue using Flotillin 1 Monoclonal antibody (67968-1-Ig)

    IHC staining of human stomach cancer using 67968-1-Ig (same clone as 67968-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 67968-1-Ig (Flotillin 1 antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67968-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human stomach cancer tissue using Flotillin 1 Monoclonal antibody (67968-1-Ig)

    IHC staining of human stomach cancer using 67968-1-Ig (same clone as 67968-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 67968-1-Ig (Flotillin 1 antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67968-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human ovary tumor tissue using Flotillin 1 Monoclonal antibody (67968-1-Ig)

    IHC staining of human ovary tumor using 67968-1-Ig (same clone as 67968-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 67968-1-Ig (Flotillin 1 antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67968-1-PBS in a different storage buffer formulation.

  • Flow cytometry (FC) experiment of Raji cells using Flotillin 1 Monoclonal antibody (67968-1-Ig)

    FC experiment of Raji using 67968-1-Ig (same clone as 67968-1-PBS)

    1x10^6 Raji cells were intracellularly stained with 0.2 ug Flotillin 1 Monoclonal antibody (67968-1-Ig, Clone:1D6C6) and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1)(red), or 0.2 ug Mouse IgG2b isotype control Mouse McAb (66360-3-Ig, Clone: 11B8C4) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 67968-1-PBS in a different storage buffer formulation.

Flotillin 1 Monoclonal antibody, PBS Only

Flotillin 1 Monoclonal Antibody for WB, FC (Intra), Indirect ELISA
Cat No. 67968-1-PBS
Clone No.1D6C6

Host / Isotype

Mouse / IgG2b

Reactivity

human, mouse, rat, pig, rabbit

Applications

WB, FC (Intra), Indirect ELISA

Synonyms

FLOT1, Flotillin-1, 1D6C6

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

67968-1-PBS targets Flotillin 1 in WB, FC (Intra), Indirect ELISA applications and shows reactivity with human, mouse, rat, pig, rabbit samples.

Tested Reactivity human, mouse, rat, pig, rabbit
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen

CatNo: Ag8694

Product name: Recombinant human Flotillin 1 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 80-427 aa of BC001146

Sequence: MLAAACQMFLGKTEAEIAHIALETLEGHQRAIMAHMTVEEIYKDRQKFSEQVFKVASSDLVNMGISVVSYTLKDIHDDQDYLHSLGKARTAQVQKDARIGEAEAKRDAGIREAKAKQEKVSAQYLSEIEMAKAQRDYELKKAAYDIEVNTRRAQADLAYQLQVAKTKQQIEEQRVQVQVVERAQQVAVQEQEIARREKELEARVRKPAEAERYKLERLAEAEKSQLIMQAEAEAASVRMRGEAEAFAIGARARAEAEQMAKKAEAFQLYQEAAQLDMLLEKLPQVAEEISGPLTSANKITLVSSGSGTMGAAKVTGEVLDILTRLPESVERLTGVSISQVNHKPLRTA

相同性解析による交差性が予測される生物種
Full Name flotillin 1
Calculated molecular weight 47 kDa
Observed molecular weight 47 kDa
GenBank accession numberBC001146
Gene Symbol Flotillin 1
Gene ID (NCBI) 10211
RRIDAB_2918719
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein A purification
UNIPROT IDO75955
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

Flotillins are membrane-associated proteins that contain two homologous isoforms, flotillin 1 (FLOT1) and flotillin 2 (FLOT2). They are highly conserved and widely expressed proteins that commonly used as markers of lipid rafts. Flotillins are involved in various signaling pathways, cell adhesion, membrane trafficking and axonal growth.

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