DDDDK tag Monoclonal antibody (Binds to FLAG® tag epitope)

DDDDK tag Monoclonal Antibody for ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

human, mouse, rat

Applications

WB, RIP, IP, IF, ELISA

Conjugate

Unconjugated

CloneNo.

2C3F4

Cat no : 66008-1-Ig

Synonyms

DDDDK tag


"DDDDK tag Antibodies" Comparison

View side-by-side comparison of DDDDK tag antibodies from other vendors to find the one that best suits your research needs.
Applications:
  • IP experiment of Transfected HEK-293 using 66008-1-Ig

    IP experiment of Transfected HEK-293 using 66008-1-Ig

    IP Result of anti-Flag tag (IP:66008-1-Ig, 3ug; Detection:66008-1-Ig 1:10000) with Transfected HEK-293 cells lysate 300ug.

  • IF Staining of Transfected HEK-293 using 66008-1-Ig

    IF Staining of Transfected HEK-293 using 66008-1-Ig

    Immunofluorescent analysis of Transfected HEK-293 cells using 66008-1-Ig (Flag tag antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Mouse IgG (H+L).


Tested Applications

Planning an IF experiment? We recommend our CoraLite® Plus 488, CoraLite®594 and CoraLite® Plus 647 conjugated versions of this antibody.

Recommended dilution

ApplicationDilution
Sample-dependent, check data in validation data gallery

Product Information

66008-1-Ig targets DDDDK tag in WB, RIP, IP, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivity human, mouse
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen Peptide 相同性解析による交差性が予測される生物種
Full Name DDDDK tag
Gene symbol
Gene ID (NCBI)
RRIDAB_2881491
Conjugate Unconjugated
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

DDDDK tag, also known as DDK tag, is a hydrophilic tag for recombinant protein technology with the sequence DYKDDDDK. The structure of DDDDK tag has been optimized for compatibility with the proteins it is attached to and unlikely to denature or inactivated them. For its high hydrophilic nature, the DDDDK tag is likely to be located on the surface of a fusion protein, which enables the tag more likely to be accessible to antibodies. This product is generated against 1xDDDDK tag (DYKDDDDK) and can recognize protein containing one or more DDDDK tags, no matter it is/they are located at N-terminal, C-terminal or at internal regions. The binding of this product with DDDDK tagged fusion protein does not depend on calcium ion. The clone of this monoclonal antibody hybridoma is 2C3F4, which is different from any other vendors.

Protocols

Product Specific Protocols
IF protocol for DDDDK tag antibody 66008-1-IgDownload protocol
IP protocol for DDDDK tag antibody 66008-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle

Nat Microbiol

Ephrin receptor A2 is an epithelial cell receptor for Epstein-Barr virus entry.

Authors - Hua Zhang
WB

Cell Metab

Receptor-Mediated ER Export of Lipoproteins Controls Lipid Homeostasis in Mice and Humans.

Authors - Xiao Wang
WB

Neuron

UBQLN2-HSP70 axis reduces poly-Gly-Ala aggregates and alleviates behavioral defects in the C9ORF72 animal model.

Authors - Kejing Zhang
WB

Plant Cell

Structural Insight into DNA Recognition by CCT/NF-YB/YC Complexes in Plant Photoperiodic Flowering.

Authors - Cuicui Shen
humanWB

Cell Death Dis

The E3 ligase TRIM26 suppresses ferroptosis through catalyzing K63-linked ubiquitination of GPX4 in glioma

Authors - Zhangjie Wang
WB

Mol Ther

Caspase-8 Induces Lysosome-Associated Cell Death in Cancer Cells.

Authors - Benfu Zhong

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Chun (Verified Customer) (07-03-2019)

This antibody is excellent

  • Applications: Western Blot,
  • Primary Antibody Dilution: 1:5000
  • Cell Tissue Type: HEK293 and HeLa cells, and primary rat cortical neurons