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Validation Data Gallery

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  • Immunohistochemistry (IHC) staining of human colon tissue using Anti-Human Fibronectin Rabbit Recombinant Antibody (98652-2-RR)

    IHC staining of human colon using 98652-2-RR (same clone as 98652-2-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 98652-2-RR (Fibronectin antibody) at dilution of 1:2000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 98652-2-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human colon tissue using Anti-Human Fibronectin Rabbit Recombinant Antibody (98652-2-RR)

    IHC staining of human colon using 98652-2-RR (same clone as 98652-2-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 98652-2-RR (Fibronectin antibody) at dilution of 1:2000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 98652-2-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of NIH/3T3 cells using Anti-Human Fibronectin Rabbit Recombinant Antibody (98652-2-RR)

    IF Staining of NIH/3T3 using 98652-2-RR (same clone as 98652-2-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed NIH/3T3 cells using Fibronectin antibody (98652-2-RR, Clone: 251989E9 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 98652-2-PBS in a different storage buffer formulation.

  • Flow cytometry (FC) experiment of MSCs(human mesenchymal stem cells) using Anti-Human Fibronectin Rabbit Recombinant Antibody (98652-2-RR)

    FC experiment of MSCs(human mesenchymal stem cells) using 98652-2-RR (same clone as 98652-2-PBS)

    1x10^6 MSCs(human mesenchymal stem cells) cells were stained with 0.25 ug Anti-Human Fibronectin Rabbit RecAb (98652-2-RR, Clone:251989E9) (red), or Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9) (blue) and APC-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were fixed and permeabilized with Intracellular Flow Cytometry Fixation & Permeabilization Buffer Kit (PF00019). This data was developed using the same antibody clone with 98652-2-PBS in a different storage buffer formulation.

  • Affinity of 98652-2-RR

    Affinity of 98652-2-RR

    Biolayer interferometry (BLl) kinetic assays of 98652-2-RR against Human Fibronectin were performed. The affinity constant is 94.5 pM.

Anti-Human Fibronectin Rabbit Recombinant Antibody, PBS Only

Fibronectin Uni-rAb® Recombinant Antibody for IHC, IF/ICC, FC(intra)
Cat No. 98652-2-PBS
Clone No.251989E9

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IHC, IF/ICC, FC(intra)

Synonyms

FN1, Anastellin, CIG, Cold insoluble globulin, Cold-insoluble globulin

Formulation:  PBS Only
PBS, Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

98652-2-PBS targets Fibronectin in IHC, IF/ICC, FC(intra) applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen

CatNo: Eg5117

Product name: Recombinant Human Fibronectin protein (rFc Tag)

Source: mammalian cells-derived, pHZ-KIsec-C-rFc

Tag: C-rFc

Domain: 607-1265 aa of NM_212482.4

Sequence: SSSGPVEVFITETPSQPNSHPIQWNAPQPSHISKYILRWRPKNSVGRWKEATIPGHLNSYTIKGLKPGVVYEGQLISIQQYGHQEVTRFDFTTTSTSTPVTSNTVTGETTPFSPLVATSESVTEITASSFVVSWVSASDTVSGFRVEYELSEEGDEPQYLDLPSTATSVNIPDLLPGRKYIVNVYQISEDGEQSLILSTSQTTAPDAPPDTTVDQVDDTSIVVRWSRPQAPITGYRIVYSPSVEGSSTELNLPETANSVTLSDLQPGVQYNITIYAVEENQESTPVVIQQETTGTPRSDTVPSPRDLQFVEVTDVKVTIMWTPPESAVTGYRVDVIPVNLPGEHGQRLPISRNTFAEVTGLSPGVTYYFKVFAVSHGRESKPLTAQQTTKLDAPTNLQFVNETDSTVLVRWTPPRAQITGYRLTVGLTRRGQPRQYNVGPSVSKYPLRNLQPASEYTVSLVAIKGNQESPKATGVFTTLQPGSSIPPYNTEVTETTIVITWTPAPRIGFKLGVRPSQGGEAPREVTSDSGSIVVSGLTPGVEYVYTIQVLRDGQERDAPIVNKVVTPLSPPTNLHLEANPDTGVLTVSWERSTTPDITGYRITTTPTNGQQGNSLEEVVHADQSSCTFDNLSPGLEYNVSVYTVKDDKESVPISDTIIP

相同性解析による交差性が予測される生物種
Full Name fibronectin 1
Calculated molecular weight272 kDa
GenBank accession numberNM_212482.4
Gene Symbol Fibronectin
Gene ID (NCBI) 2335
RRIDAB_3746249
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein A purification
UNIPROT IDP02751-15
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

Fibronectin 1 (FN1) is a high molecular weight glycoprotein which exists in both a soluble form in plasma (plasma FN1) and other body fluids and an insoluble form in the extracellular matrix (cellular FN1). Plasma FN1 (dimeric form) is secreted by hepatocytes. Cellular FN (dimeric or cross-linked multimeric forms), made by fibroblasts, epithelial and other cell types, is deposited as fibrils in the extracellular matrix. FN1 binds to cell surfaces through integrins and to various compounds including collagen, fibrin and heparin. It is involved in cell adhesion and migration processes including embryogenesis, wound healing, hemostasis, host defense, and metastasis. (PMID: 7276612; 12244123; 12847088)

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