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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using Fibronectin Recombinant monoclonal antibody (80911-4-RR)

    WB analysis using 80911-4-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 80911-4-RR (Fibronectin antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of NIH/3T3 cells using Fibronectin Recombinant monoclonal antibody (80911-4-RR)

    WB analysis of NIH/3T3 using 80911-4-RR

    NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 80911-4-RR (Fibronectin antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of rat plasma using Fibronectin Recombinant monoclonal antibody (80911-4-RR)

    WB analysis of rat plasma using 80911-4-RR

    rat plasma were subjected to SDS PAGE followed by western blot with 80911-4-RR (Fibronectin antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of NIH/3T3 cells using Fibronectin Recombinant monoclonal antibody (80911-4-RR)

    IF Staining of NIH/3T3 using 80911-4-RR

    Immunofluorescent analysis of (-20°C Ethanol) fixed NIH/3T3 cells using Fibronectin antibody (80911-4-RR, Clone: 251989E12 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

Fibronectin Recombinant monoclonal antibody

Fibronectin Uni-rAb® Recombinant Antibody for WB, IF/ICC, ELISA
Cat No. 80911-4-RR
Clone No.251989E12

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF/ICC, ELISA

Synonyms

FN1, Anastellin, CIG, Cold insoluble globulin, Cold-insoluble globulin

Formulation:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Unconjugated
CoraLite® Plus 488
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inNIH/3T3 cells, HepG2 cells, rat plasma, mouse uterus tissue
Positive IF/ICC detected inNIH/3T3 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

80911-4-RR targets Fibronectin in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen

CatNo: Eg5117

Product name: Recombinant Human Fibronectin protein (rFc Tag)

Source: mammalian cells-derived, pHZ-KIsec-C-rFc

Tag: C-rFc

Domain: 607-1265 aa of NM_212482.4

Sequence: SSSGPVEVFITETPSQPNSHPIQWNAPQPSHISKYILRWRPKNSVGRWKEATIPGHLNSYTIKGLKPGVVYEGQLISIQQYGHQEVTRFDFTTTSTSTPVTSNTVTGETTPFSPLVATSESVTEITASSFVVSWVSASDTVSGFRVEYELSEEGDEPQYLDLPSTATSVNIPDLLPGRKYIVNVYQISEDGEQSLILSTSQTTAPDAPPDTTVDQVDDTSIVVRWSRPQAPITGYRIVYSPSVEGSSTELNLPETANSVTLSDLQPGVQYNITIYAVEENQESTPVVIQQETTGTPRSDTVPSPRDLQFVEVTDVKVTIMWTPPESAVTGYRVDVIPVNLPGEHGQRLPISRNTFAEVTGLSPGVTYYFKVFAVSHGRESKPLTAQQTTKLDAPTNLQFVNETDSTVLVRWTPPRAQITGYRLTVGLTRRGQPRQYNVGPSVSKYPLRNLQPASEYTVSLVAIKGNQESPKATGVFTTLQPGSSIPPYNTEVTETTIVITWTPAPRIGFKLGVRPSQGGEAPREVTSDSGSIVVSGLTPGVEYVYTIQVLRDGQERDAPIVNKVVTPLSPPTNLHLEANPDTGVLTVSWERSTTPDITGYRITTTPTNGQQGNSLEEVVHADQSSCTFDNLSPGLEYNVSVYTVKDDKESVPISDTIIP

相同性解析による交差性が予測される生物種
Full Name fibronectin 1
Calculated molecular weight272 kDa
Observed molecular weight220-275 kDa
GenBank accession numberNM_212482.4
Gene Symbol Fibronectin
Gene ID (NCBI) 2335
RRIDAB_3743358
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein A purification
UNIPROT IDP02751-15
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Fibronectin (fibronectin) is a kind of macromolecular glycoprotein widely existing in extracellular matrix (ECM), plasma and various cell surfaces, which plays a central role in key biological processes such as cell adhesion, migration, proliferation, differentiation, tissue repair and embryo development. Fibronectin is usually a dimer structure, which consists of two subunits connected by disulfide bonds. There are two main forms of Fibronectin: plasma Fibronectin(pFN) is synthesized by liver cells and exists in the blood, which is involved in the processes of coagulation and wound repair. Cellular Fibronectin(cFN) is synthesized by fibroblasts and other cells, deposited in extracellular matrix, and participates in the interaction between cells and matrix. Its molecular weight ranges from 220 to 275 kDa.

Protocols

Product Specific Protocols
IF protocol for Fibronectin antibody 80911-4-RRDownload protocol
WB protocol for Fibronectin antibody 80911-4-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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