Validation Data Gallery
Tested Applications
| Positive WB detected in | HeLa cells |
| Positive IHC detected in | human stomach cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunohistochemistry (IHC) | IHC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
83836-4-RR targets Fibrillin 1 in WB, IHC, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Recombinant |
| Type | Antibody |
| Immunogen |
CatNo: Ag30283 Product name: Recombinant human FBN1 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 367-462 aa of BC146854 Sequence: SPGVTVAPEMCPIRATEDFNKLCSVPMVIPGRPEYPPPPLGPIPPVLPVPPGFPPGPQIPVPRPPVEYLYPSREPPRVLPVNVTDYCQLVRYLCQN 相同性解析による交差性が予測される生物種 |
| Full Name | fibrillin 1 |
| Observed molecular weight | 350 kDa |
| GenBank accession number | BC146854 |
| Gene Symbol | Fibrillin 1 |
| Gene ID (NCBI) | 2200 |
| RRID | AB_3671419 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Protein A purfication |
| UNIPROT ID | P35555 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
Fibrillin-1 belongs to the fibrillin family. Fibrillins are structural components of 10-12 nm extracellular calcium-binding microfibrils, which occur either in association with elastin or in elastin-free bundles. Fibrillin-1-containing microfibrils provide long-term force bearing structural support. Defects in Fibrillin-1 are a cause of Marfan syndrome (MFS), isolated ectopia lentis (EL), Weill-Marchesani syndrome autosomal dominant (ADWMS), Shprintzen-Goldberg craniosynostosis syndrome (SGS) or MASS syndrome.
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for Fibrillin 1 antibody 83836-4-RR | Download protocol |
| WB protocol for Fibrillin 1 antibody 83836-4-RR | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
