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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using Fibrillarin Recombinant monoclonal antibody (87432-2-RR)

    WB analysis using 87432-2-RR (same clone as 87432-2-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 87432-2-RR (FBL antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 87432-2-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using Fibrillarin Recombinant monoclonal antibody (87432-2-RR)

    IF Staining of HepG2 using 87432-2-RR (same clone as 87432-2-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using Fibrillarin antibody (87432-2-RR, Clone: 252802F8 ) at dilution of 1:1000 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 87432-2-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of NIH/3T3 cells using Fibrillarin Recombinant monoclonal antibody (87432-2-RR)

    IF Staining of NIH/3T3 using 87432-2-RR (same clone as 87432-2-PBS)

    Immunofluorescent analysis of (4% PFA) fixed NIH/3T3 cells using Fibrillarin antibody (87432-2-RR, Clone: 252802F8 ) at dilution of 1:1000 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 87432-2-PBS in a different storage buffer formulation.

Fibrillarin Recombinant monoclonal antibody, PBS Only

Fibrillarin Uni-rAb® Recombinant Antibody for WB, IF/ICC, Indirect ELISA
Cat No. 87432-2-PBS
Clone No.252802F8

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IF/ICC, Indirect ELISA

Synonyms

FBL, EC:2.1.1.-, FIB, FIB1, FLRN

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

87432-2-PBS targets Fibrillarin in WB, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen

CatNo: Ag8841

Product name: Recombinant human FBL protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 1-321 aa of BC019260

Sequence: MKPGFSPRGGGFGGRGGFGDRGGRGGRGGFGGGRGRGGGFRGRGRGGGGGGGGGGGGGRGGGGFHSGGNRGRGRGGKRGNQSGKNVMVEPHRHEGVFICRGKEDALVTKNLVPGESVYGEKRVSISEGDDKIEYRAWNPFRSKLAAAILGGVDQIHIKPGAKVLYLGAASGTTVSHVSDIVGPDGLVYAVEFSHRSGRDLINLAKKRTNIIPVIEDARHPHKYRMLIAMVDVIFADVAQPDQTRIVALNAHTFLRNGGHFVISIKANCIDSTASAEAVFASEVKKMQQENMKPQEQLTLEPYERDHAVVVGVYRPPPKVKN

相同性解析による交差性が予測される生物種
Full Name fibrillarin
Calculated molecular weight 321 aa, 34 kDa
Observed molecular weight 34 kDa
GenBank accession numberBC019260
Gene Symbol FBL
Gene ID (NCBI) 2091
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein A purification
UNIPROT IDP22087
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

Fibrillarin (Fbl) is an essential nucleolar protein that plays an essential role in ribosome biogenesis and more particularly in the methylation of ribosomal RNAs and rDNA histones. FBL is highly expressed in various cancers and correlates with poor survival outcomes in cancer patients,such as hepatocellular carcinoma(PMID: 33376525). Knockdown of FBL sensitizes tumor cells and xenografts to DNA crosslinking agents, and leads to homologous recombination-mediated DNA repair defects(PMID: 37489617).

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