Validation Data Gallery

Filter: AllWBIHCIF

WB analysis of A549 using 68068-1-Ig (same clone as 68068-1-PBS)
A549 cells were subjected to SDS PAGE followed by western blot with 68068-1-Ig (Ferritin light chain antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68068-1-PBS in a different storage buffer formulation.
WB analysis of HeLa using 68068-1-Ig (same clone as 68068-1-PBS)
HeLa cells were subjected to SDS PAGE followed by western blot with 68068-1-Ig (Ferritin light chain antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68068-1-PBS in a different storage buffer formulation.
WB analysis of HepG2 using 68068-1-Ig (same clone as 68068-1-PBS)
HepG2 cells were subjected to SDS PAGE followed by western blot with 68068-1-Ig (Ferritin light chain antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68068-1-PBS in a different storage buffer formulation.
WB analysis of human placenta using 68068-1-Ig (same clone as 68068-1-PBS)
human placenta tissue were subjected to SDS PAGE followed by western blot with 68068-1-Ig (Ferritin light chain antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68068-1-PBS in a different storage buffer formulation.
IHC staining of mouse brain using 68068-1-Ig (same clone as 68068-1-PBS)
Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 68068-1-Ig (Ferritin light chain antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68068-1-PBS in a different storage buffer formulation.
IHC staining of mouse brain using 68068-1-Ig (same clone as 68068-1-PBS)
Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 68068-1-Ig (Ferritin light chain antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68068-1-PBS in a different storage buffer formulation.
IHC staining of human prostate cancer using 68068-1-Ig (same clone as 68068-1-PBS)
Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 68068-1-Ig (Ferritin light chain antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68068-1-PBS in a different storage buffer formulation.
IHC staining of human prostate cancer using 68068-1-Ig (same clone as 68068-1-PBS)
Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 68068-1-Ig (Ferritin light chain antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68068-1-PBS in a different storage buffer formulation.
IHC staining of human liver cancer using 68068-1-Ig (same clone as 68068-1-PBS)
Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 68068-1-Ig (Ferritin light chain antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68068-1-PBS in a different storage buffer formulation.
IHC staining of human liver cancer using 68068-1-Ig (same clone as 68068-1-PBS)
Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 68068-1-Ig (Ferritin light chain antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68068-1-PBS in a different storage buffer formulation.
IHC staining of human colon cancer using 68068-1-Ig (same clone as 68068-1-PBS)
Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 68068-1-Ig (Ferritin light chain antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68068-1-PBS in a different storage buffer formulation.
IHC staining of human colon cancer using 68068-1-Ig (same clone as 68068-1-PBS)
Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 68068-1-Ig (Ferritin light chain antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68068-1-PBS in a different storage buffer formulation.
IF Staining of HepG2 using 68068-1-Ig (same clone as 68068-1-PBS)
Immunofluorescent analysis of (-20°C Methanol) fixed HepG2 cells using FTL antibody (68068-1-Ig, Clone: 1F9F5 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 68068-1-PBS in a different storage buffer formulation.

Ferritin light chain Monoclonal antibody, PBS Only

Ferritin light chain Monoclonal Antibody for WB, IHC, IF/ICC, ELISA

Cat No. 68068-1-PBS

Clone No.1F9F5

Host / Isotype

Mouse / IgG1

Reactivity

Human, Mouse

Applications

WB, IHC, IF/ICC, ELISA

Synonyms

Ferritin L subunit, Ferritin light chain, ferritin, light polypeptide, FTL

Formulation: PBS Only

PBS, Azide, Glycerol

PBS Only

Conjugate: Unconjugated

Size/Concentration:

-/ -

ご購入について

国内販売は「コスモ・バイオ株式会社」を通じて行っております。お見積り・ご注文はお近くの販売代理店へご連絡ください。

国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター（国内）に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。

保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Overview

Tested Applications
Product Information

データシート［PDF］を印刷

MSDS

Tested Applications

Recommended dilution

Application	Dilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

68068-1-PBS targets Ferritin light chain in WB, IHC, IF/ICC, ELISA applications and shows reactivity with Human, Mouse samples.

Tested Reactivity	Human, Mouse
Host / Isotype	Mouse / IgG1
Class	Monoclonal
Type	Antibody
Immunogen	CatNo: Ag20637 Product name: Recombinant human FTL protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 1-175 aa of BC008439 Sequence: MSSQIRQNYSTDVEAAVNSLVNLYLQASYTYLSLGFYFDRDDVALEGVSHFFRELAEEKREGYERLLKMQNQRGGRALFQDIKKPAEDEWGKTPDAMKAAMALEKKLNQALLDLHALGSARTDPHLCDFLETHFLDEEVKLIKKMGDHLTNLHRLGGPEAGLGEYLFERLTLKHD 相同性解析による交差性が予測される生物種
Full Name	ferritin, light polypeptide
Calculated molecular weight	19 kDa
Observed molecular weight	18 kDa
GenBank accession number	BC008439
Gene Symbol	Ferritin light chain
Gene ID (NCBI)	2512
RRID	AB_2918809
Conjugate	Unconjugated
Form
Form	Liquid
Purification Method	Protein G purification
UNIPROT ID	P02792
Storage Buffer	PBS only{{ptg:BufferTemp}}7.3
Storage Conditions	Store at -80°C.

Background Information

FTL belongs to the ferritin family. It stores iron in a soluble, non-toxic, readily available form. FTL is important for iron homeostasis. It plays a role in delivery of iron to cells. FTL mediates iron uptake in capsule cells of the developing kidney. Mutation of FTL will cause hereditary hyperferritinemia-cataract syndrome (HHCS) or neurodegeneration with brain iron accumulation type 3 (NBIA3). Ferritin light polypeptide (FTL) and ferritin heavy polypeptide (FTH1) were the main constituents the striatum and cerebellar cortex revealed.

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Validation Data Gallery

WB analysis of A549 using 68068-1-Ig (same clone as 68068-1-PBS)

WB analysis of HeLa using 68068-1-Ig (same clone as 68068-1-PBS)

WB analysis of HepG2 using 68068-1-Ig (same clone as 68068-1-PBS)

WB analysis of human placenta using 68068-1-Ig (same clone as 68068-1-PBS)

IHC staining of mouse brain using 68068-1-Ig (same clone as 68068-1-PBS)