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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using Ferritin heavy chain Polyclonal antibody (11682-1-AP)

    WB analysis using 11682-1-AP (same clone as 11682-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 11682-1-AP (Ferritin heavy chain antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11682-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HeLa cells using Ferritin heavy chain Polyclonal antibody (11682-1-AP)

    WB analysis of HeLa using 11682-1-AP (same clone as 11682-1-PBS)

    WB result of Ferritin heavy chain antibody (11682-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-Ferritin heavy chain transfected HeLa cells. This data was developed using the same antibody clone with 11682-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using Ferritin heavy chain Polyclonal antibody (11682-1-AP)

    WB analysis using 11682-1-AP (same clone as 11682-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 11682-1-AP (Ferritin heavy chain antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11682-1-PBS in a different storage buffer formulation.

  • Immunoprecipitation (IP) experiment of HeLa cells using Ferritin heavy chain Polyclonal antibody (11682-1-AP)

    IP experiment of HeLa using 11682-1-AP (same clone as 11682-1-PBS)

    IP result of anti-Ferritin heavy chain (IP:11682-1-AP, 4ug; Detection:11682-1-AP 1:3000) with HeLa cells lysate 1600 ug. This data was developed using the same antibody clone with 11682-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of rat liver tissue using Ferritin heavy chain Polyclonal antibody (11682-1-AP)

    IHC staining of rat liver using 11682-1-AP (same clone as 11682-1-PBS)

    Immunohistochemical analysis of paraffin-embedded rat liver tissue slide using 11682-1-AP (Ferritin heavy chain antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 11682-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver tissue using Ferritin heavy chain Polyclonal antibody (11682-1-AP)

    IHC staining of human liver using 11682-1-AP (same clone as 11682-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 11682-1-AP (Ferritin heavy chain antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 11682-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of rat liver tissue using Ferritin heavy chain Polyclonal antibody (11682-1-AP)

    IHC staining of rat liver using 11682-1-AP (same clone as 11682-1-PBS)

    Immunohistochemical analysis of paraffin-embedded rat liver tissue slide using 11682-1-AP (Ferritin heavy chain antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 11682-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver tissue using Ferritin heavy chain Polyclonal antibody (11682-1-AP)

    IHC staining of human liver using 11682-1-AP (same clone as 11682-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 11682-1-AP (Ferritin heavy chain antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 11682-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse liver tissue using Ferritin heavy chain Polyclonal antibody (11682-1-AP)

    IHC staining of mouse liver using 11682-1-AP (same clone as 11682-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 11682-1-AP (Ferritin heavy chain antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 11682-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse liver tissue using Ferritin heavy chain Polyclonal antibody (11682-1-AP)

    IHC staining of mouse liver using 11682-1-AP (same clone as 11682-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 11682-1-AP (Ferritin heavy chain antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 11682-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using Ferritin heavy chain Polyclonal antibody (11682-1-AP)

    IF Staining of HeLa using 11682-1-AP (same clone as 11682-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using Ferritin heavy chain antibody (11682-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 11682-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using Ferritin heavy chain Polyclonal antibody (11682-1-AP)

    IF Staining of HeLa using 11682-1-AP (same clone as 11682-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using Ferritin heavy chain antibody (11682-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 11682-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO Validated

Ferritin heavy chain Polyclonal antibody, PBS Only

Ferritin heavy chain Polyclonal Antibody for WB, IHC, IF/ICC, IP, Indirect ELISA
Cat No. 11682-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, Indirect ELISA

Synonyms

Ferritin, FTH1, Cell proliferation-inducing gene 15 protein, EC:1.16.3.1, Ferritin heavy chain, N-terminally processed

Formulation:  PBS Only
PBS, Azide, Glycerol
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

11682-1-PBS targets Ferritin heavy chain in WB, IHC, IF/ICC, IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag2290

Product name: Recombinant human FTH1 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-183 aa of BC000857

Sequence: MTTASTSQVRQNYHQDSEAAINRQINLELYASYVYLSMSYYFDRDDVALKNFAKYFLHQSHEEREHAEKLMKLQNQRGGRIFLQDIKKPDCDDWESGLNAMECALHLEKNVNQSLLELHKLATDKNDPHLCDFIETHYLNEQVKAIKELGDHVTNLRKMGAPESGLAEYLFDKHTLGDSDNES

相同性解析による交差性が予測される生物種
Full Name ferritin, heavy polypeptide 1
Calculated molecular weight 183 aa, 21 kDa
Observed molecular weight 21 kDa
GenBank accession numberBC000857
Gene Symbol FTH1
Gene ID (NCBI) 2495
RRIDAB_3669131
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDP02794
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

Ferritin heavy chain(FTH1, ferritin, heavy polypeptide 1 ), as a 21 kDa subunit of the ferritin complex, exhibits ferroxidase activity, which serves an essential role in catalyzing the conversion of the ferrous ions (Fe2+) to the ferric form (Fe3+). FTH1 regulates angiogenesis during inflammation and malignancy by binding to high molecular weight kininogen (HKa) and blocking its anti-angiogenic activity on endothelial cells in vitro and in vivo(PMID: 33260500). FTH1 has been shown to protect proximal tube epithelial cells and kidneys against the activity of free iron in reactive oxygen species generation. FTH1 plays an important role in the maintenance of the cellular iron balance in ferroptosis.

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