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Validation Data Gallery

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  • Western Blot (WB) analysis of human plasma using Factor XIIIb Polyclonal antibody (20269-1-AP)

    WB analysis of human plasma using 20269-1-AP

    human plasma were subjected to SDS PAGE followed by western blot with 20269-1-AP (Factor XIIIb antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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Factor XIIIb Polyclonal antibody

Factor XIIIb Polyclonal Antibody for WB, ELISA
Cat No. 20269-1-AP
Publications(1)

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Synonyms

Transglutaminase B chain, Protein-glutamine gamma-glutamyltransferase B chain, FXIII-B, FXIIIB, Fibrin-stabilizing factor B subunit

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Positive WB detected inhuman plasma

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

20269-1-AP targets Factor XIIIb in WB, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide 相同性解析による交差性が予測される生物種
Full Name coagulation factor XIII, B polypeptide
Calculated molecular weight 76 kDa
Observed molecular weight~80 kDa
GenBank accession numberNM_001994
Gene Symbol Factor XIIIb
Gene ID (NCBI) 2165
RRIDAB_3085601
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP05160
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Coagulation factor XIII (FXIII) is a blood proenzyme that catalyzes intermolecular cross-linking of fibrinogen and is involved in the clotting cascade. Factor XIII is composed of two catalytic FXIIIa and two protective/regulatory FXIIIb subunits. FXIIIb is the carrier/regulatory protein of its partner FXIIIa subunit.

Protocols

Product Specific Protocols
WB protocol for Factor XIIIb antibody 20269-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Biomol Biomed

F13B regulates angiogenesis and tumor progression in hepatocellular carcinoma via the HIF-1α/VEGF pathway

Authors - Dong Jiang
  • KO Validated
View Article