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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using FYB Polyclonal antibody (31553-1-AP)

    WB analysis using 31553-1-AP (same clone as 31553-1-PBS)

    Jurkat cells were subjected to SDS PAGE followed by western blot with 31553-1-AP (FYB antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 31553-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human ovary cancer tissue using FYB Polyclonal antibody (31553-1-AP)

    IHC staining of human ovary cancer using 31553-1-AP (same clone as 31553-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 31553-1-AP (FYB antibody) at dilution of 1:500 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 31553-1-PBS in a different storage buffer formulation.

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FYB Polyclonal antibody, PBS Only

FYB Polyclonal Antibody for WB, IHC, Indirect ELISA
Cat No. 31553-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, Indirect ELISA

Synonyms

SLAP130, SLAP 130, p120/p130, FYB 120/130, ADAP

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

31553-1-PBS targets FYB in WB, IHC, Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen FYB fusion protein Ag35929 相同性解析による交差性が予測される生物種
Full Name FYN binding protein (FYB-120/130)
Calculated molecular weight85kDa，783aa
Observed molecular weight110 kDa
GenBank accession numberNM_199335.3
Gene Symbol FYB
Gene ID (NCBI) 2533
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity Purification
UNIPROT IDO15117
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

FYN binding protein (FYB-120/130), also known as FYB, ADAP (Adhesion and degranulation-promoting adapter protein), and SLAP-130 (SLP-76-associated phosphoprotein) is a protein that is encoded by the FYB gene in humans. The protein is highly expressed in T cells, NK cells, bone marrow cells, and platelets, responsible for the signal transduction upon T cell receptor (TCR) stimulation with integrin activation.