Validation Data Gallery
Tested Applications
| Positive WB detected in | pig brain tissue, rat brain tissue, mouse brain tissue, fetal human brain tissue |
| Positive IHC detected in | human cervical cancer tissue, human breast cancer tissue, human stomach tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF-P detected in | human breast cancer tissue |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)-P | IF-P : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 2 publications below |
| WB | See 2 publications below |
| IHC | See 1 publications below |
Product Information
60230-1-Ig targets FUT9 in WB, IHC, IF-P, ELISA applications and shows reactivity with human, pig, mouse, rat samples.
| Tested Reactivity | human, pig, mouse, rat |
| Cited Reactivity | human, mouse |
| Host / Isotype | Mouse / IgG2a |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag8298 Product name: Recombinant human FUT9 protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 32-359 aa of BC036101 Sequence: PTNSWIFSPMESASSVLKMKNFFSTKTDYFNETTILVWVWPFGQTFDLTSCQAMFNIQGCHLTTDRSLYNKSHAVLIHHRDISWDLTNLPQQARPPFQKWIWMNLESPTHTPQKSGIEHLFNLTLTYRRDSDIQVPYGFLTVSTNPFVFEVPSKEKLVCWVVSNWNPEHARVKYYNELSKSIEIHTYGQAFGEYVNDKNLIPTISACKFYLSFENSIHKDYITEKLYNAFLAGSVPVVLGPSRENYENYIPADSFIHVEDYNSPSELAKYLKEVDKNNKLYLSYFNWRKDFTVNLPRFWESHACLACDHVKRHQEYKSVGNLEKWFWN 相同性解析による交差性が予測される生物種 |
| Full Name | fucosyltransferase 9 (alpha (1,3) fucosyltransferase) |
| Calculated molecular weight | 359 aa, 42 kDa |
| Observed molecular weight | 44-46 kDa |
| GenBank accession number | BC036101 |
| Gene Symbol | FUT9 |
| Gene ID (NCBI) | 10690 |
| RRID | AB_11183046 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | Q9Y231 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
FUT9 is the main enzyme responsible for the synthesis of Lewis X (Lex) and catalyzes the last step in the biosynthesis of Lewis X antigen by addition of a fucose residue to precursor glycan structures. FUT9 is expressed in stomach, kidney, brain, and in leukocytes.
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for FUT9 antibody 60230-1-Ig | Download protocol |
| IHC protocol for FUT9 antibody 60230-1-Ig | Download protocol |
| WB protocol for FUT9 antibody 60230-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Cancers (Basel) FUT9-Driven Programming of Colon Cancer Cells towards a Stem Cell-Like State.
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Acta Biochim Biophys Sin (Shanghai) ELF4 contributes to esophageal squamous cell carcinoma growth and metastasis by augmenting cancer stemness via FUT9
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