Validation Data Gallery

Filter: AllWBIHC

WB analysis of MDA-MB-231 using 66118-1-Ig (same clone as 66118-1-PBS)
MDA-MB-231 cells were subjected to SDS PAGE followed by western blot with 66118-1-Ig (FUT8 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66118-1-PBS in a different storage buffer formulation.
WB analysis of MCF-7 using 66118-1-Ig (same clone as 66118-1-PBS)
WB result of FUT8 antibody (66118-1-Ig; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-FUT8 transfected MCF-7 cells. This data was developed using the same antibody clone with 66118-1-PBS in a different storage buffer formulation.
WB analysis of MCF-7 using 66118-1-Ig (same clone as 66118-1-PBS)
MCF-7 cells were subjected to SDS PAGE followed by western blot with 66118-1-Ig (FUT8 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66118-1-PBS in a different storage buffer formulation.
WB analysis of Caco-2 using 66118-1-Ig (same clone as 66118-1-PBS)
Caco-2 cells were subjected to SDS PAGE followed by western blot with 66118-1-Ig (FUT8 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66118-1-PBS in a different storage buffer formulation.
WB analysis of MDA-MB-453s using 66118-1-Ig (same clone as 66118-1-PBS)
MDA-MB-453s cells were subjected to SDS PAGE followed by western blot with 66118-1-Ig (FUT8 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66118-1-PBS in a different storage buffer formulation.
IHC staining of human colon cancer using 66118-1-Ig (same clone as 66118-1-PBS)
Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 66118-1-Ig (FUT8 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66118-1-PBS in a different storage buffer formulation.
IHC staining of human colon cancer using 66118-1-Ig (same clone as 66118-1-PBS)
Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 66118-1-Ig (FUT8 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66118-1-PBS in a different storage buffer formulation.
IHC staining of mouse kidney using 66118-1-Ig (same clone as 66118-1-PBS)
Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 66118-1-Ig (FUT8 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66118-1-PBS in a different storage buffer formulation.
IHC staining of mouse cerebellum using 66118-1-Ig (same clone as 66118-1-PBS)
Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 66118-1-Ig (FUT8 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66118-1-PBS in a different storage buffer formulation.
IHC staining of human breast cancer using 66118-1-Ig (same clone as 66118-1-PBS)
Immunohistochemical analysis of paraffin-embedded human breast cancer slide using 66118-1-Ig (FUT8 Antibody) at dilution of 1:50 under 40x lens. This data was developed using the same antibody clone with 66118-1-PBS in a different storage buffer formulation.
IHC staining of human lung cancer using 66118-1-Ig (same clone as 66118-1-PBS)
Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 66118-1-Ig (FUT8 Antibody) at dilution of 1:50 under 10x lens. This data was developed using the same antibody clone with 66118-1-PBS in a different storage buffer formulation.
IHC staining of human lung cancer using 66118-1-Ig (same clone as 66118-1-PBS)
Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 66118-1-Ig (FUT8 Antibody) at dilution of 1:50 under 40x lens. This data was developed using the same antibody clone with 66118-1-PBS in a different storage buffer formulation.
IHC staining of human breast cancer using 66118-1-Ig (same clone as 66118-1-PBS)
Immunohistochemical analysis of paraffin-embedded human breast cancer slide using 66118-1-Ig (FUT8 Antibody) at dilution of 1:50 under 10x lens. This data was developed using the same antibody clone with 66118-1-PBS in a different storage buffer formulation.

Featured Product
KD/KO Validated

FUT8 Monoclonal antibody, PBS Only

FUT8 Monoclonal Antibody for WB, IHC, Indirect ELISA

Cat No. 66118-1-PBS

Clone No.2F7H2

Host / Isotype

Mouse / IgG2a

Reactivity

human, mouse

Applications

WB, IHC, Indirect ELISA

Synonyms

2F7H2, Alpha-(1,6)-fucosyltransferase, alpha1 6FucT, Alpha1-6FucT, EC:2.4.1.68

Formulation: PBS Only

PBS, Azide, Glycerol

PBS Only

Conjugate: Unconjugated

Size/Concentration:

-/ -

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Overview

Tested Applications
Product Information

データシート［PDF］を印刷

MSDS

Tested Applications

Recommended dilution

Application	Dilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

66118-1-PBS targets FUT8 in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity	human, mouse
Host / Isotype	Mouse / IgG2a
Class	Monoclonal
Type	Antibody
Immunogen	CatNo: Ag18769 Product name: Recombinant human FUT8 protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 276-575 aa of BC025385 Sequence: HWSGEVKDKNVQVVEFPIVDSLHPRPPYLPLAVPEDLADRLVRVHGDPAVWWVSQFVKYLIRPQPWLEKEIEEATKKLGFKHPVIGVHVRRTDKVGTEAAFHPIEEYMVHVEEHFQLLARRMQVDKKRVYLATDDPSLLKEAKTKYPNYEFISDNSISWSAGLHNRYTENSLRGVILDIHFLSQADFLVCTFSSQVCRVAYEIMQTLHPDASANFHSLDDIYYFGGQNAHNQIAIYAHQPRTADEIPMEPGDIIGVAGNHWDGYSKGVNRKLGRTGLYPSYKVREKIETVKYPTYPEAEK 相同性解析による交差性が予測される生物種
Full Name	fucosyltransferase 8 (alpha (1,6) fucosyltransferase)
Calculated molecular weight	66 kDa
Observed molecular weight	66 kDa
GenBank accession number	BC025385
Gene Symbol	FUT8
Gene ID (NCBI)	2530
RRID	AB_2881517
Conjugate	Unconjugated
Form
Form	Liquid
Purification Method	Protein A purification
UNIPROT ID	Q9BYC5
Storage Buffer	PBS only{{ptg:BufferTemp}}7.3
Storage Conditions	Store at -80°C.

Background Information

Fucosyltransferase 8 (FUT8) is GDP-fucose-glycoprotein fucosyltransferase and is localized in Golgi apparatus. FUT8 is involved in protein glycosylation by catalyzing the addition of fucose in alpha 1-6 linkage to the first GlcNAc residue, next to the peptide chains in N-glycans. FUT8 may be involved in human aging by regulating protein N-glycosylation and in the molecular pathogenesis of human pulmonary emphysema (PE).

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Validation Data Gallery

WB analysis of MDA-MB-231 using 66118-1-Ig (same clone as 66118-1-PBS)

WB analysis of MCF-7 using 66118-1-Ig (same clone as 66118-1-PBS)

WB analysis of MCF-7 using 66118-1-Ig (same clone as 66118-1-PBS)

WB analysis of Caco-2 using 66118-1-Ig (same clone as 66118-1-PBS)

WB analysis of MDA-MB-453s using 66118-1-Ig (same clone as 66118-1-PBS)

IHC staining of human colon cancer using 66118-1-Ig (same clone as 66118-1-PBS)