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Validation Data Gallery

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  • Western Blot (WB) analysis of human placenta tissue using FUT3 Monoclonal antibody (67344-1-Ig)

    WB analysis of human placenta using 67344-1-Ig

    human placenta tissue were subjected to SDS PAGE followed by western blot with 67344-1-Ig (FUT3 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of NCCIT cells using FUT3 Monoclonal antibody (67344-1-Ig)

    WB analysis of NCCIT using 67344-1-Ig

    NCCIT cells were subjected to SDS PAGE followed by western blot with 67344-1-Ig (FUT3 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of JAR cells using FUT3 Monoclonal antibody (67344-1-Ig)

    WB analysis of JAR using 67344-1-Ig

    JAR cells were subjected to SDS PAGE followed by western blot with 67344-1-Ig (FUT3 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HepG2 cells using FUT3 Monoclonal antibody (67344-1-Ig)

    WB analysis of HepG2 using 67344-1-Ig

    HepG2 cells were subjected to SDS PAGE followed by western blot with 67344-1-Ig (FUT3 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of JAR cells using FUT3 Monoclonal antibody (67344-1-Ig)

    WB analysis of JAR using 67344-1-Ig

    JAR cells were subjected to SDS PAGE followed by western blot with 67344-1-Ig (FUT3 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using FUT3 Monoclonal antibody (67344-1-Ig)

    IHC staining of human colon cancer using 67344-1-Ig

    Immunohistochemical analysis of paraffin-embedded colon cancer slide using 67344-1-Ig (FUT3 antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using FUT3 Monoclonal antibody (67344-1-Ig)

    IHC staining of human colon cancer using 67344-1-Ig

    Immunohistochemical analysis of paraffin-embedded colon cancer slide using 67344-1-Ig (FUT3 antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human prostate hyperplasia tissue using FUT3 Monoclonal antibody (67344-1-Ig)

    IHC staining of human prostate hyperplasia using 67344-1-Ig

    Immunohistochemical analysis of paraffin-embedded human prostate hyperplasia tissue slide using 67344-1-Ig (FUT3 antibody) at dilution of 1:500 (under 10x lens)..

  • Immunohistochemistry (IHC) staining of human prostate hyperplasia tissue using FUT3 Monoclonal antibody (67344-1-Ig)

    IHC staining of human prostate hyperplasia using 67344-1-Ig

    Immunohistochemical analysis of paraffin-embedded human prostate hyperplasia tissue slide using 67344-1-Ig (FUT3 antibody) at dilution of 1:500 (under 40x lens)..

  • Immunofluorescence (IF) / fluorescent staining of human prostate hyperplasia tissue using FUT3 Monoclonal antibody (67344-1-Ig)

    IF Staining of human prostate hyperplasia using 67344-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed human prostate hyperplasia tissue using FUT3 antibody (67344-1-Ig, Clone: 2A6G7 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

  • Immunofluorescence (IF) / fluorescent staining of human prostate hyperplasia tissue using FUT3 Monoclonal antibody (67344-1-Ig)

    IF Staining of human prostate hyperplasia using 67344-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed human prostate hyperplasia tissue using FUT3 antibody (67344-1-Ig, Clone: 2A6G7 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

FUT3 Monoclonal antibody

FUT3 Monoclonal Antibody for WB, IHC, IF-P, ELISA
Cat No. 67344-1-Ig
Clone No.2A6G7

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

WB, IHC, IF-P, ELISA

Synonyms

3-galactosyl-N-acetylglucosaminide 4-alpha-L-fucosyltransferase FUT3, 4-galactosyl-N-acetylglucosaminide 3-alpha-L-fucosyltransferase, Alpha-3-fucosyltransferase FUT3, Blood group Lewis alpha-4-fucosyltransferase, CD174

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inhuman placenta tissue, NCCIT cells, JAR cells, HepG2 cells
Positive IHC detected inhuman colon cancer tissue, human prostate hyperplasia tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected inhuman prostate hyperplasia tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
Immunofluorescence (IF)-PIF-P : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

67344-1-Ig targets FUT3 in WB, IHC, IF-P, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen

CatNo: Ag12581

Product name: Recombinant human FUT3 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 84-361 aa of BC108675

Sequence: MVPGTADCHITADRKVYPQADTVIVHHWDIMSNPKSRLPPSPRPQGQRWIWFNLEPPPNCQHLEALDRYFNLTMSYRSDSDIFTPYGWLEPWSGQPAHPPLNLSAKTELVAWAVSNWKPDSARVRYYQSLQAHLKVDVYGRSHKPLPKGTMMETLSRYKFYLAFENSLHPDYITEKLWRNALEAWAVPVVLGPSRSNYERFLPPDAFIHVDDFQSPKDLARYLQELDKDHARYLSYFRWRETLRPRSFSWALDFCKACWKLQQESRYQTVRSIAAWFT

相同性解析による交差性が予測される生物種
Full Name fucosyltransferase 3 (galactoside 3(4)-L-fucosyltransferase, Lewis blood group)
Calculated molecular weight 361 aa, 42 kDa
Observed molecular weight 42-45 kDa
GenBank accession numberBC108675
Gene Symbol FUT3
Gene ID (NCBI) 2525
RRIDAB_2882602
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein G purification
UNIPROT IDP21217
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for FUT3 antibody 67344-1-IgDownload protocol
IHC protocol for FUT3 antibody 67344-1-IgDownload protocol
IF protocol for FUT3 antibody 67344-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols