Validation Data Gallery
Tested Applications
| Positive WB detected in | A549 cells, Calu-3 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
20288-1-AP targets FUT2 in WB, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag14047 Product name: Recombinant human FUT2 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 29-153 aa of BC001899 Sequence: QRLAKIQAMWELPVQIPVLASTSKALGPSQLRGMWTINAIGRLGNQMGEYATLYALAKMNGRPAFIPAQMHSTLAPIFRITLPVLHSATASRIPWQNYHLNDWMEEEYRHIPGEYVRFTGYPCS 相同性解析による交差性が予測される生物種 |
| Full Name | fucosyltransferase 2 (secretor status included) |
| Calculated molecular weight | 343 aa, 39 kDa |
| Observed molecular weight | 35-39 kDa |
| GenBank accession number | BC001899 |
| Gene Symbol | FUT2 |
| Gene ID (NCBI) | 2524 |
| RRID | AB_3669331 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q10981 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
Fucosyltransferase 2 (FUT2) is a key enzyme that catalyzes the transfer of fucose to the terminal galactose of type 1 or type 2 disaccharide via α1,2-linkage. FUT2 gene codes for the alpha(1,2)fucosyltransferase responsible for the synthesis of the H antigen, which is the precursor of the ABO histo-blood group antigens in body fluids and on the intestinal mucosa (PMID: 19487333). FUT2 is highly expressed in lung adenocarcinoma (LUAD) and plays a vital role in the tumorigenesis of LUAD (PMID: 36552800). FUT2 has a calculated molecular mass of ~39 kDa, and the 70kDa band might be the glycosylated form.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for FUT2 antibody 20288-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |