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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

FTSJD2 Polyclonal antibody

FTSJD2 Polyclonal Antibody for WB, IF, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human, Mouse

Applications

WB, IF, IHC, ELISA

Conjugate

Unconjugated

Cat no : 27707-1-AP

Synonyms

CMTR1, FTSJD2, hMTr1, ISG95, KIAA0082, MTr1

Validation Data Gallery

Applications:
  • WB analysis using 27707-1-AP

    WB analysis using 27707-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 27707-1-AP (FTSJD2 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  • IHC staining of human testis using 27707-1-AP

    IHC staining of human testis using 27707-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 27707-1-AP (FTSJD2 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human testis using 27707-1-AP

    IHC staining of human testis using 27707-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 27707-1-AP (FTSJD2 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse testis using 27707-1-AP

    IHC staining of mouse testis using 27707-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 27707-1-AP (FTSJD2 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse testis using 27707-1-AP

    IHC staining of mouse testis using 27707-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 27707-1-AP (FTSJD2 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of U2OS using 27707-1-AP

    IF Staining of U2OS using 27707-1-AP

    Immunofluorescent analysis of (4% PFA) fixed U2OS cells using 27707-1-AP (FTSJD2 antibody) at dilution of 1:50 and CoraLite488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Tested Applications

Positive WB detected inU-251 cells, NIH/3T3 cells
Positive IHC detected inhuman testis tissue, mouse testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inU2OS cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

27707-1-AP targets FTSJD2 in WB, IF, IHC, ELISA applications and shows reactivity with Human, Mouse samples.

Tested Reactivity Human, Mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen FTSJD2 fusion protein Ag26722 相同性解析による交差性が予測される生物種
Full Name FtsJ methyltransferase domain containing 2
Calculated molecular weight 835 aa, 95 kDa
Observed molecular weight 96-100 kDa
GenBank accession numberBC031890
Gene symbol FTSJD2
Gene ID (NCBI) 23070
RRIDAB_2880949
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for FTSJD2 antibody 27707-1-APDownload protocol
IHC protocol for FTSJD2 antibody 27707-1-APDownload protocol
IF protocol for FTSJD2 antibody 27707-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols