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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using FTSJ3 Recombinant antibody (84311-6-RR)

    WB analysis using 84311-6-RR (same clone as 84311-6-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 84311-6-RR (FTSJ3 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84311-6-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of MCF-7 cells using FTSJ3 Recombinant antibody (84311-6-RR)

    IF Staining of MCF-7 using 84311-6-RR (same clone as 84311-6-PBS)

    Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using FTSJ3 antibody (84311-6-RR, Clone: 241507F2 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 84311-6-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 84311-6-RR

    Affinity and Kinetic Characterization of 84311-6-RR

    Biolayer interferometry (BLl) kinetic assays of 84311-6-RR against Human FTSJ3 were performed. The affinity constant is 0.68 nM.

FTSJ3 Recombinant monoclonal antibody, PBS Only

FTSJ3 Uni-rAb® Recombinant Antibody for WB, IF/ICC, Indirect ELISA
Cat No. 84311-6-PBS
Clone No.241507F2

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, Indirect ELISA

Synonyms

SB92, pre-rRNA 2'-O-ribose RNA methyltransferase FTSJ3, FTSJ 3, EPCS3, EC:2.1.1.-

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

84311-6-PBS targets FTSJ3 in WB, IF/ICC, Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen

CatNo: Ag9835

Product name: Recombinant human FTSJ3 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 491-847 aa of BC036710

Sequence: KRMRLTEVQDDKEEEEEENPLLVPLEEKAVLQEEQANLWFSKGSFAGIEDDADEALEISQAQLLFENRRKGRQQQQKQQLPQTPPSCLKTEIMSPLYQDEAPKGTEASSGTEAATGLEGEEKDGISDSDSSTSSEEEESWEPLRGKKRSRGPKSDDDGFEIVPIEDPAKHRILDPEGLALGAVIASSKKAKRDLIDNSFNRYTFNEDEGELPEWFVQEEKQHRIRQLPVGKKEVEHYRKRWREINARPIKKVAEAKARKKRRMLKRLEQTRKKAEAVVNTVDISEREKVAQLRSLYKKAGLGKEKRHVTYVVAKKGVGRKVRRPAGVRGHFKVVDSRMKKDQRAQQRKEQKKKHKRK

相同性解析による交差性が予測される生物種
Full Name FtsJ homolog 3 (E. coli)
Calculated molecular weight 847 aa, 97 kDa
Observed molecular weight120-130 kDa
GenBank accession numberBC036710
Gene Symbol FTSJ3
Gene ID (NCBI) 117246
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein A purfication
UNIPROT IDQ8IY81
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

FTSJ3 is an RNA 2'-O-methyltransferase recruited by HIV to avoid innate immune sensing.

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