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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using Ferritin heavy chain Monoclonal antibody (60875-5-Ig)

    WB analysis using 60875-5-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 60875-5-Ig (Ferritin heavy chain antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using Ferritin heavy chain Monoclonal antibody (60875-5-Ig)

    WB analysis using 60875-5-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 60875-5-Ig (Ferritin heavy chain antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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Ferritin heavy chain Monoclonal antibody

Ferritin heavy chain Monoclonal Antibody for WB, ELISA
Cat No. 60875-5-Ig
Clone No.3G8D7

Host / Isotype

Mouse / IgG2a

Reactivity

human, mouse, rat, rabbit

Applications

WB, ELISA

Synonyms

Ferritin, FTH1, Cell proliferation-inducing gene 15 protein, EC:1.16.3.1, Ferritin heavy chain, N-terminally processed

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inA549 cells, rabbit brain tissue, HeLa cells, K-562 cells, rat brain tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

60875-5-Ig targets Ferritin heavy chain in WB, ELISA applications and shows reactivity with human, mouse, rat, rabbit samples.

Tested Reactivity human, mouse, rat, rabbit
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen Ferritin heavy chain fusion protein Ag21386 相同性解析による交差性が予測される生物種
Full Name ferritin, heavy polypeptide 1
Calculated molecular weight 183 aa, 21 kDa
Observed molecular weight 21 kDa
GenBank accession numberBC000857
Gene Symbol FTH1
Gene ID (NCBI) 2495
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP02794
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Ferritin heavy chain(FTH1, ferritin, heavy polypeptide 1 ), as a 21 kDa subunit of the ferritin complex, exhibits ferroxidase activity, which serves an essential role in catalyzing the conversion of the ferrous ions (Fe2+) to the ferric form (Fe3+). FTH1 regulates angiogenesis during inflammation and malignancy by binding to high molecular weight kininogen (HKa) and blocking its anti-angiogenic activity on endothelial cells in vitro and in vivo(PMID: 33260500). FTH1 has been shown to protect proximal tube epithelial cells and kidneys against the activity of free iron in reactive oxygen species generation. FTH1 plays an important role in the maintenance of the cellular iron balance in ferroptosis.

Protocols

Product Specific Protocols
WB protocol for Ferritin heavy chain antibody 60875-5-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols