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Validation Data Gallery

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  • Western Blot (WB) analysis of mouse liver tissue using FTCD Monoclonal antibody (66979-1-Ig)

    WB analysis of mouse liver using 66979-1-Ig (same clone as 66979-1-PBS)

    mouse liver tissue were subjected to SDS PAGE followed by western blot with 66979-1-Ig (FTCD antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66979-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of pig kidney tissue using FTCD Monoclonal antibody (66979-1-Ig)

    WB analysis of pig kidney using 66979-1-Ig (same clone as 66979-1-PBS)

    pig kidney tissue were subjected to SDS PAGE followed by western blot with 66979-1-Ig (FTCD antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66979-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of pig liver tissue using FTCD Monoclonal antibody (66979-1-Ig)

    WB analysis of pig liver using 66979-1-Ig (same clone as 66979-1-PBS)

    pig liver tissue were subjected to SDS PAGE followed by western blot with 66979-1-Ig (FTCD antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66979-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of rat liver tissue using FTCD Monoclonal antibody (66979-1-Ig)

    WB analysis of rat liver using 66979-1-Ig (same clone as 66979-1-PBS)

    rat liver tissue were subjected to SDS PAGE followed by western blot with 66979-1-Ig (FTCD antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66979-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human kidney tissue using FTCD Monoclonal antibody (66979-1-Ig)

    IHC staining of human kidney using 66979-1-Ig (same clone as 66979-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 66979-1-Ig (FTCD antibody) at dilution of 1:300 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66979-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human kidney tissue using FTCD Monoclonal antibody (66979-1-Ig)

    IHC staining of human kidney using 66979-1-Ig (same clone as 66979-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 66979-1-Ig (FTCD antibody) at dilution of 1:300 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66979-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using FTCD Monoclonal antibody (66979-1-Ig)

    IHC staining of human liver cancer using 66979-1-Ig (same clone as 66979-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66979-1-Ig (FTCD antibody) at dilution of 1:300 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66979-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using FTCD Monoclonal antibody (66979-1-Ig)

    IHC staining of human liver cancer using 66979-1-Ig (same clone as 66979-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66979-1-Ig (FTCD antibody) at dilution of 1:300 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66979-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of human liver cancer tissue using FTCD Monoclonal antibody (66979-1-Ig)

    IF Staining of human liver cancer using 66979-1-Ig (same clone as 66979-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed human liver cancer tissue using FTCD antibody (66979-1-Ig, Clone: 1H8A1 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66979-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of human liver cancer tissue using FTCD Monoclonal antibody (66979-1-Ig)

    IF Staining of human liver cancer using 66979-1-Ig (same clone as 66979-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed human liver cancer tissue using FTCD antibody (66979-1-Ig, Clone: 1H8A1 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66979-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of mouse kidney tissue using FTCD Monoclonal antibody (66979-1-Ig)

    IF Staining of mouse kidney using 66979-1-Ig (same clone as 66979-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse kidney tissue using FTCD antibody (66979-1-Ig, Clone: 1H8A1 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66979-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO Validated

FTCD Monoclonal antibody, PBS Only

FTCD Monoclonal Antibody for WB, IHC, IF-P, Indirect ELISA
Cat No. 66979-1-PBS
Clone No.1H8A1

Host / Isotype

Mouse / IgG2b

Reactivity

human, mouse, rat, pig

Applications

WB, IHC, IF-P, Indirect ELISA

Synonyms

Formimidoyltetrahydrofolate cyclodeaminase, Formimidoyltransferase-cyclodeaminase, Formiminotransferase-cyclodeaminase, Glutamate formimidoyltransferase, Glutamate formiminotransferase

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

66979-1-PBS targets FTCD in WB, IHC, IF-P, Indirect ELISA applications and shows reactivity with human, mouse, rat, pig samples.

Tested Reactivity human, mouse, rat, pig
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen

CatNo: Ag17429

Product name: Recombinant human FTCD protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 192-541 aa of BC136395

Sequence: TKEQAHRIALNLREQGRGKDQPGRLKKVQGIGWYLDEKNLAQVSTNLLDFEVTALHTVYEETCREAQELSLPVVGSQLVGLVPLKALLDAAAFYCEKENLFILEEEQRIRLVVSRLGLDSLCPFSPKERIIEYLVPERGPERGLGSKSLRAFVGEVGARSAAPGGGSVAAAAAAMGAALGSMVGLMTYGRRQFQSLDTTMRRLIPPFREASAKLTTLVDADAEAFTAYLEAMRLPKNTPEEKDRRTAALQEGLRRAVSVPLTLAETVASLWPALQELARCGNLACRSDLQVAAKALEMGVFGAYFNVLINLRDITDEAFKDQIHHRVSSLLQEAKTQAALVLDCLETRQE

相同性解析による交差性が予測される生物種
Full Name formiminotransferase cyclodeaminase
Calculated molecular weight 541 aa, 59 kDa
Observed molecular weight 53-62 kDa
GenBank accession numberBC136395
Gene Symbol FTCD
Gene ID (NCBI) 10841
RRIDAB_2882299
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein A purification
UNIPROT IDO95954
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

FTCD (formimidoyltransferase cyclodeaminase) is critical for the catabolism of histidine. This process is one-carbon units that can enter the one-carbon/folate cycle, which provides methyl groups for arsenic metabolism (PMID: 30893314). In addition, FTCD could serve as a predictive and prognostic marker for patients with HCC (PMID: 37675273).