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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

FSD2 Polyclonal antibody

FSD2 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 25609-1-AP

Synonyms

FSD2, RP11 127F21, SPRYD1

Validation Data Gallery

Applications:
  • WB analysis of mouse heart using 25609-1-AP

    WB analysis of mouse heart using 25609-1-AP

    mouse heart tissue were subjected to SDS PAGE followed by western blot with 25609-1-AP (FSD2 Antibody) at dilution of 1:600 incubated at 4 degree celsius over night.

  • WB analysis using 25609-1-AP

    WB analysis using 25609-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 25609-1-AP (FSD2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • WB analysis of rat heart using 25609-1-AP

    WB analysis of rat heart using 25609-1-AP

    rat heart tissue were subjected to SDS PAGE followed by western blot with 25609-1-AP (FSD2 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse heart using 25609-1-AP

    IHC staining of mouse heart using 25609-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 25609-1-AP (FSD2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with .

  • IHC staining of mouse heart using 25609-1-AP

    IHC staining of mouse heart using 25609-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 25609-1-AP (FSD2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with .

Tested Applications

Positive WB detected inmouse heart tissue, rat heart tissue
Positive IHC detected inmouse heart tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

25609-1-AP targets FSD2 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen FSD2 fusion protein Ag22229 相同性解析による交差性が予測される生物種
Full Name fibronectin type III and SPRY domain containing 2
Calculated molecular weight 749 aa, 85 kDa
Observed molecular weight 95-105 kDa
GenBank accession numberBC130569
Gene symbol FSD2
Gene ID (NCBI) 123722
RRIDAB_2880159
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for FSD2 antibody 25609-1-APDownload protocol
IHC protocol for FSD2 antibody 25609-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

FH

Zee (Verified Customer) (01-28-2020)

It worked very well with western blot.

  • Applications: Western Blot,
  • Primary Antibody Dilution: 1:1000
  • Cell Tissue Type: Mouse heart tissue