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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using FRYL Polyclonal antibody (24085-1-AP)

    WB analysis using 24085-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 24085-1-AP (FRYL antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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FRYL Polyclonal antibody

FRYL Polyclonal Antibody for WB, ELISA
Cat No. 24085-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, ELISA

Synonyms

Protein furry homolog like, KIAA0826, FRY like, AF4P12

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inDU 145 cells, HEK-293 cells, HeLa cells, U2OS cells, NIH/3T3 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

24085-1-AP targets FRYL in WB, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen FRYL fusion protein Ag21333 相同性解析による交差性が予測される生物種
Full Name FRY-like
Calculated molecular weight 3013 aa, 340 kDa
Observed molecular weight340 kDa
GenBank accession numberBC021803
Gene Symbol FRYL
Gene ID (NCBI) 285527
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen Affinity purified
UNIPROT IDO94915
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

FRYL, or FRY-like transcription coactivator, is a protein that belongs to the Furry protein family, which is evolutionarily conserved from yeast to humans. FRYL has almost identical functional domains to the well-characterized Fry protein, which is involved in many processes, including cell polarization, regulation of the actin cytoskeleton, and patterning of sensory neuron dendritic fields (PMID: 34386489). FRYL is a prognostic marker in certain cancers, including Kidney renal clear cell carcinoma, Ovary serous cystadenocarcinoma, and Rectum adenocarcinoma, suggesting its potential role in cancer progression.

Protocols

Product Specific Protocols
WB protocol for FRYL antibody 24085-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols